Orthopedic implant particle-induced tumor necrosis factor-α production in macrophage-monocyte lineage cells is mediated by nuclear factor of activated T cells

Hiroshi Minematsu, Mike J. Shin, Ayse B. Celil Aydemir, Wook Seo Sung, Won Kim Dae, Theodore A. Blaine, Fernando Macian-Juan, Jay Yang, Francis Young In Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Wear particles produced from artificial joint prostheses are known to cause macrophage-monocyte lineage cells to produce proosteoclastogenic cytokines, including tumor necrosis factor (TNF)-α. The specific molecular mechanism, however, is not yet known. Bioinformatic analysis showed that the promoter region of TNF-α has several consensus sequences for NFAT binding. Consequently, we examined the role of nuclear factor of activated T cells (NFAT) in TNF-α production. Our investigation has shown that treatment with titanium nanoparticles increased TNF-α gene expression along with TNF-α protein secretion in murine macrophage-like RAW264.7 and primary monocyte-macrophage cells. Titanium particle-induced TNF-α induction was inhibited by VIVIT, a peptide inhibitor that targets the calcineurin/NFAT axis, which suggests that NFAT mediates metallic particle-induced TNF-α expression in monocyte-macrophage lineage cells.

Original languageEnglish (US)
Pages (from-to)143-150
Number of pages8
JournalAnnals of the New York Academy of Sciences
Volume1117
DOIs
StatePublished - Nov 2007

Fingerprint

NFATC Transcription Factors
Macrophages
Orthopedics
Monocytes
Tumor Necrosis Factor-alpha
Titanium
Joint prostheses
Joint Prosthesis
Calcineurin
Consensus Sequence
Bioinformatics
Particle
Cells
Computational Biology
Genetic Promoter Regions
Gene expression
Nanoparticles
Wear of materials
Cytokines
Gene Expression

Keywords

  • Macrophage
  • NFAT
  • Osteolysis
  • TNF-α
  • Wear particle

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Orthopedic implant particle-induced tumor necrosis factor-α production in macrophage-monocyte lineage cells is mediated by nuclear factor of activated T cells. / Minematsu, Hiroshi; Shin, Mike J.; Celil Aydemir, Ayse B.; Sung, Wook Seo; Dae, Won Kim; Blaine, Theodore A.; Macian-Juan, Fernando; Yang, Jay; Lee, Francis Young In.

In: Annals of the New York Academy of Sciences, Vol. 1117, 11.2007, p. 143-150.

Research output: Contribution to journalArticle

Minematsu, Hiroshi ; Shin, Mike J. ; Celil Aydemir, Ayse B. ; Sung, Wook Seo ; Dae, Won Kim ; Blaine, Theodore A. ; Macian-Juan, Fernando ; Yang, Jay ; Lee, Francis Young In. / Orthopedic implant particle-induced tumor necrosis factor-α production in macrophage-monocyte lineage cells is mediated by nuclear factor of activated T cells. In: Annals of the New York Academy of Sciences. 2007 ; Vol. 1117. pp. 143-150.
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abstract = "Wear particles produced from artificial joint prostheses are known to cause macrophage-monocyte lineage cells to produce proosteoclastogenic cytokines, including tumor necrosis factor (TNF)-α. The specific molecular mechanism, however, is not yet known. Bioinformatic analysis showed that the promoter region of TNF-α has several consensus sequences for NFAT binding. Consequently, we examined the role of nuclear factor of activated T cells (NFAT) in TNF-α production. Our investigation has shown that treatment with titanium nanoparticles increased TNF-α gene expression along with TNF-α protein secretion in murine macrophage-like RAW264.7 and primary monocyte-macrophage cells. Titanium particle-induced TNF-α induction was inhibited by VIVIT, a peptide inhibitor that targets the calcineurin/NFAT axis, which suggests that NFAT mediates metallic particle-induced TNF-α expression in monocyte-macrophage lineage cells.",
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AU - Sung, Wook Seo

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AU - Macian-Juan, Fernando

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