Origin of endothelial progenitors in human postnatal bone marrow

Morayma Reyes, Arkadiusz Dudek, Balkrishna Jahagirdar, Lisa Koodie, Paul H. Marker, Catherine M. Verfaillie

Research output: Contribution to journalArticle

1091 Scopus citations

Abstract

This study demonstrates that a CD34-, vascular endothelial cadherin- (VE-cadherin-), AC133+, and fetal liver kinase+ (Flk1+) multipotent adult progenitor cell (MAPC) that copurifies with mesenchymal stem cells from postnatal human bone marrow (BM) is a progenitor for angioblasts. In vitro, MAPCs cultured with VEGF differentiate into CD34+, VE-cadherin+, Flk1+ cells - a phenotype that would be expected for angioblasts. They subsequently differentiate into cells that express endothelial markers, function in vitro as mature endothelial cells, and contribute to neoangiogenesis in vivo during tumor angiogenesis and wound healing. This in vitro model of preangioblast-to-endothelium differentiation should prove very useful in studying commitment to the angioblast and beyond. In vivo, MAPCs can differentiate in response to local cues into endothelial cells that contribute to neoangiogenesis in tumors. Because MAPCs can be expanded in culture without obvious senescence for more than 80 population doublings, they may be an important source of endothelial cells for cellular pro- or anti-angiogenic therapies.

Original languageEnglish (US)
Pages (from-to)337-346
Number of pages10
JournalJournal of Clinical Investigation
Volume109
Issue number3
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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    Reyes, M., Dudek, A., Jahagirdar, B., Koodie, L., Marker, P. H., & Verfaillie, C. M. (2002). Origin of endothelial progenitors in human postnatal bone marrow. Journal of Clinical Investigation, 109(3), 337-346. https://doi.org/10.1172/JCI0214327