Organizational principles of the connexin-related brain transcriptome

David C. Spray, Dumitru A. Iacobas

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We have found that deletion of genes encoding the gap junction proteins Cx43, Cx32 and Cx36 alter the expression levels of large numbers of genes in mouse brain located on all chromosomes and encoding proteins from all major functional categories. Gene regulation in Cx32 and Cx43 null brains was more similar than that in the Cx36 null brain, suggesting the possibility of transcriptomic controls exerted by both genes on both astrocytes and oligodendrocytes. In order to explore the nature of expression linkage among the genes, we examined coordinated expression patterns in wild-type and connexin null brains. Coordination with Cx43 in wild-type brain predicted regulation in Cx43 nulls with considerable accuracy. Moreover, interlinkage within gene networks was greatly perturbed in the Cx43 null brain. These findings suggest several principles regarding regulatory transcriptomic networks involving gap junction genes and raise the issue of the underlying cause of connexin null phenotypes as well as mechanisms of regulation.

Original languageEnglish (US)
Pages (from-to)39-47
Number of pages9
JournalJournal of Membrane Biology
Volume218
Issue number1-3
DOIs
StatePublished - Aug 2007

Fingerprint

Connexins
Connexin 43
Transcriptome
Brain
Genes
Gene Regulatory Networks
Gap Junctions
Oligodendroglia
Gene Deletion
Astrocytes
Chromosomes
Phenotype
Proteins

Keywords

  • Astrocyte
  • Cx32
  • Cx36
  • Cx43
  • Gap junction
  • Gene expression
  • Glia
  • Oligodendrocyte
  • Panglial syncytium

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

Cite this

Organizational principles of the connexin-related brain transcriptome. / Spray, David C.; Iacobas, Dumitru A.

In: Journal of Membrane Biology, Vol. 218, No. 1-3, 08.2007, p. 39-47.

Research output: Contribution to journalArticle

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