Order of genes on human chromosome 5q with respect to 5q interstitial deletions

K. Huebner, L. Nagarajan, E. Besa, E. Angert, B. J. Lange, L. A. Cannizzaro, H. Van Den Berghe, D. Santoli, J. Finan, C. M. Croce, P. C. Nowell

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Abstract

Using (a) somatic cell hybrids retaining partial chromosome 5 and (b) clinical samples from patients with acquired deletions of the long arm of chromosome 5, combined with chromosome 5-linked DNA probes, some of which exhibited RFLPs, we have determined the order of a series of genes on chromosome 5. The order established is 5pter→MLVI-2→cen-→HEXB→DHFR→Pi227→cp12.6→ (IL5,IL4)→IL3→GMCSF→FGFA→ (CSF1R,PDGFR)→(treC,ADRBR)→(ARH-H9,CSF1)→qter. The suggested order and orientation for the closely linked IL3/GMCSF gene pair is cen→5′ IL3 3′→5′ GMCSF 3′→qter, on the basis of analysis of the GMCSF rearrangement in HL60 DNA. The map position of the GRL locus, which was consistent with both somatic cell hybrid and 5q- analyses, was telomeric to GMCSF and centromeric to CSF1R/PDGFR, near FGFA. Long-range restriction-enzyme analysis of 5q- DNAs did not detect rearrangements of 5q-linked probes except in HL60 DNA, but it did reveal putative long-range RFLPs of several loci. RFLPs for GRL, Pi227, cp12.6, IL3, and CSF1R can detect deletions in bone marrow and in leukemia cells from patients with acquired 5q deletions.

Original languageEnglish (US)
Pages (from-to)26-36
Number of pages11
JournalAmerican Journal of Human Genetics
Volume46
Issue number1
StatePublished - Jan 1990
Externally publishedYes

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Chromosomes, Human, Pair 5
Gene Order
Human Chromosomes
Restriction Fragment Length Polymorphisms
Hybrid Cells
DNA
Restriction Mapping
Interleukin-5
DNA Probes
Interleukin-4
Genes
Leukemia
Bone Marrow

ASJC Scopus subject areas

  • Genetics

Cite this

Huebner, K., Nagarajan, L., Besa, E., Angert, E., Lange, B. J., Cannizzaro, L. A., ... Nowell, P. C. (1990). Order of genes on human chromosome 5q with respect to 5q interstitial deletions. American Journal of Human Genetics, 46(1), 26-36.

Order of genes on human chromosome 5q with respect to 5q interstitial deletions. / Huebner, K.; Nagarajan, L.; Besa, E.; Angert, E.; Lange, B. J.; Cannizzaro, L. A.; Van Den Berghe, H.; Santoli, D.; Finan, J.; Croce, C. M.; Nowell, P. C.

In: American Journal of Human Genetics, Vol. 46, No. 1, 01.1990, p. 26-36.

Research output: Contribution to journalArticle

Huebner, K, Nagarajan, L, Besa, E, Angert, E, Lange, BJ, Cannizzaro, LA, Van Den Berghe, H, Santoli, D, Finan, J, Croce, CM & Nowell, PC 1990, 'Order of genes on human chromosome 5q with respect to 5q interstitial deletions', American Journal of Human Genetics, vol. 46, no. 1, pp. 26-36.
Huebner K, Nagarajan L, Besa E, Angert E, Lange BJ, Cannizzaro LA et al. Order of genes on human chromosome 5q with respect to 5q interstitial deletions. American Journal of Human Genetics. 1990 Jan;46(1):26-36.
Huebner, K. ; Nagarajan, L. ; Besa, E. ; Angert, E. ; Lange, B. J. ; Cannizzaro, L. A. ; Van Den Berghe, H. ; Santoli, D. ; Finan, J. ; Croce, C. M. ; Nowell, P. C. / Order of genes on human chromosome 5q with respect to 5q interstitial deletions. In: American Journal of Human Genetics. 1990 ; Vol. 46, No. 1. pp. 26-36.
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abstract = "Using (a) somatic cell hybrids retaining partial chromosome 5 and (b) clinical samples from patients with acquired deletions of the long arm of chromosome 5, combined with chromosome 5-linked DNA probes, some of which exhibited RFLPs, we have determined the order of a series of genes on chromosome 5. The order established is 5pter→MLVI-2→cen-→HEXB→DHFR→Pi227→cp12.6→ (IL5,IL4)→IL3→GMCSF→FGFA→ (CSF1R,PDGFR)→(treC,ADRBR)→(ARH-H9,CSF1)→qter. The suggested order and orientation for the closely linked IL3/GMCSF gene pair is cen→5′ IL3 3′→5′ GMCSF 3′→qter, on the basis of analysis of the GMCSF rearrangement in HL60 DNA. The map position of the GRL locus, which was consistent with both somatic cell hybrid and 5q- analyses, was telomeric to GMCSF and centromeric to CSF1R/PDGFR, near FGFA. Long-range restriction-enzyme analysis of 5q- DNAs did not detect rearrangements of 5q-linked probes except in HL60 DNA, but it did reveal putative long-range RFLPs of several loci. RFLPs for GRL, Pi227, cp12.6, IL3, and CSF1R can detect deletions in bone marrow and in leukemia cells from patients with acquired 5q deletions.",
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