TY - JOUR
T1 - Order of genes on human chromosome 5q with respect to 5q interstitial deletions
AU - Huebner, K.
AU - Nagarajan, L.
AU - Besa, E.
AU - Angert, E.
AU - Lange, B. J.
AU - Cannizzaro, L. A.
AU - Van Den Berghe, H.
AU - Santoli, D.
AU - Finan, J.
AU - Croce, C. M.
AU - Nowell, P. C.
PY - 1990/1
Y1 - 1990/1
N2 - Using (a) somatic cell hybrids retaining partial chromosome 5 and (b) clinical samples from patients with acquired deletions of the long arm of chromosome 5, combined with chromosome 5-linked DNA probes, some of which exhibited RFLPs, we have determined the order of a series of genes on chromosome 5. The order established is 5pter→MLVI-2→cen-→HEXB→DHFR→Pi227→cp12.6→ (IL5,IL4)→IL3→GMCSF→FGFA→ (CSF1R,PDGFR)→(treC,ADRBR)→(ARH-H9,CSF1)→qter. The suggested order and orientation for the closely linked IL3/GMCSF gene pair is cen→5′ IL3 3′→5′ GMCSF 3′→qter, on the basis of analysis of the GMCSF rearrangement in HL60 DNA. The map position of the GRL locus, which was consistent with both somatic cell hybrid and 5q- analyses, was telomeric to GMCSF and centromeric to CSF1R/PDGFR, near FGFA. Long-range restriction-enzyme analysis of 5q- DNAs did not detect rearrangements of 5q-linked probes except in HL60 DNA, but it did reveal putative long-range RFLPs of several loci. RFLPs for GRL, Pi227, cp12.6, IL3, and CSF1R can detect deletions in bone marrow and in leukemia cells from patients with acquired 5q deletions.
AB - Using (a) somatic cell hybrids retaining partial chromosome 5 and (b) clinical samples from patients with acquired deletions of the long arm of chromosome 5, combined with chromosome 5-linked DNA probes, some of which exhibited RFLPs, we have determined the order of a series of genes on chromosome 5. The order established is 5pter→MLVI-2→cen-→HEXB→DHFR→Pi227→cp12.6→ (IL5,IL4)→IL3→GMCSF→FGFA→ (CSF1R,PDGFR)→(treC,ADRBR)→(ARH-H9,CSF1)→qter. The suggested order and orientation for the closely linked IL3/GMCSF gene pair is cen→5′ IL3 3′→5′ GMCSF 3′→qter, on the basis of analysis of the GMCSF rearrangement in HL60 DNA. The map position of the GRL locus, which was consistent with both somatic cell hybrid and 5q- analyses, was telomeric to GMCSF and centromeric to CSF1R/PDGFR, near FGFA. Long-range restriction-enzyme analysis of 5q- DNAs did not detect rearrangements of 5q-linked probes except in HL60 DNA, but it did reveal putative long-range RFLPs of several loci. RFLPs for GRL, Pi227, cp12.6, IL3, and CSF1R can detect deletions in bone marrow and in leukemia cells from patients with acquired 5q deletions.
UR - http://www.scopus.com/inward/record.url?scp=0025139915&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025139915&partnerID=8YFLogxK
M3 - Article
C2 - 2294753
AN - SCOPUS:0025139915
SN - 0002-9297
VL - 46
SP - 26
EP - 36
JO - American Journal of Human Genetics
JF - American Journal of Human Genetics
IS - 1
ER -