Orbiting of Flagellated Bacteria within a Thin Fluid Film around Micrometer-Sized Particles

George Araujo, Weijie Chen, Sridhar Mani, Jay X. Tang

Research output: Contribution to journalArticle

Abstract

Bacterial motility under confinement is relevant to both environmental control and the spread of infection. Here, we report observations on Escherichia coli, Enterobacter sp., Pseudomonas aeruginosa, and Bacillus subtilis when they are confined within a thin layer of water around dispersed micrometer-sized particles sprinkled over a semisolid agar gel. In this setting, E. coli and Enterobacteria orbit around the dispersed particles. The liquid layer is shaped like a shallow tent with its height at the center set by the seeding particle, and the meniscus profile set by the strong surface tension of water. The tent-shaped confinement and the left handedness of the flagellar filaments result in exclusively clockwise circular trajectories. The thin fluid layer is resilient because of a balance between evaporation and reinforcement of fluid that permeated out of the agar. The latter is driven by the Laplace pressure caused by the concave meniscus. In short, we explain the physical mechanism of a convenient method to entrap bacteria within localized thin fluid film near a permeable surface.

Original languageEnglish (US)
JournalBiophysical Journal
DOIs
StatePublished - Jan 1 2019

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Agar
Escherichia coli
Bacteria
Enterobacter
Functional Laterality
Surface Tension
Water
Orbit
Enterobacteriaceae
Infection Control
Bacillus subtilis
Pseudomonas aeruginosa
Gels
Pressure
Meniscus
Reinforcement (Psychology)

ASJC Scopus subject areas

  • Biophysics

Cite this

Orbiting of Flagellated Bacteria within a Thin Fluid Film around Micrometer-Sized Particles. / Araujo, George; Chen, Weijie; Mani, Sridhar; Tang, Jay X.

In: Biophysical Journal, 01.01.2019.

Research output: Contribution to journalArticle

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