Optogenetically controlled protein kinases for regulation of cellular signaling

Anna V. Leopold, Konstantin G. Chernov, Vladislav Verkhusha

Research output: Contribution to journalReview article

6 Citations (Scopus)

Abstract

Protein kinases are involved in the regulation of many cellular processes including cell differentiation, survival, migration, axon guidance and neuronal plasticity. A growing set of optogenetic tools, termed opto-kinases, allows activation and inhibition of different protein kinases with light. The optogenetic regulation enables fast, reversible and non-invasive manipulation of protein kinase activities, complementing traditional methods, such as treatment with growth factors, protein kinase inhibitors or chemical dimerizers. In this review, we summarize the properties of the existing optogenetic tools for controlling tyrosine kinases and serine-threonine kinases. We discuss how the opto-kinases can be applied for studies of spatial and temporal aspects of protein kinase signaling in cells and organisms. We compare approaches for chemical and optogenetic regulation of protein kinase activity and present guidelines for selection of opto-kinases and equipment to control them with light. We also describe strategies to engineer novel opto-kinases on the basis of various photoreceptors.

Original languageEnglish (US)
Pages (from-to)2454-2484
Number of pages31
JournalChemical Society Reviews
Volume47
Issue number7
DOIs
StatePublished - Apr 7 2018

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Cell signaling
Protein Kinases
Phosphotransferases
Protein-Serine-Threonine Kinases
Protein Kinase Inhibitors
Protein-Tyrosine Kinases
Plasticity
Intercellular Signaling Peptides and Proteins
Chemical activation
Engineers

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Optogenetically controlled protein kinases for regulation of cellular signaling. / Leopold, Anna V.; Chernov, Konstantin G.; Verkhusha, Vladislav.

In: Chemical Society Reviews, Vol. 47, No. 7, 07.04.2018, p. 2454-2484.

Research output: Contribution to journalReview article

Leopold, Anna V. ; Chernov, Konstantin G. ; Verkhusha, Vladislav. / Optogenetically controlled protein kinases for regulation of cellular signaling. In: Chemical Society Reviews. 2018 ; Vol. 47, No. 7. pp. 2454-2484.
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