Optimization of neuropeptide extraction from the mouse hypothalamus

Fa Yun Che, Xin Zhang, Iryna Berezniuk, Myrasol Callaway, Jihyeon Lim, Lloyd D. Fricker

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Sample preparation for neuropeptidomic studies is a critical issue since protein degradation can produce high levels of peptides that obscure the endogenous neuropeptides. We compared different extraction conditions for the recovery of neuropeptides and the formation of protein breakdown fragments from mouse hypothalami. Sonication and heating in water (70°C for 20 min) followed by cold acid and centrifugation enabled the efficient extraction of many neuropeptides without the formation of protein degradation fragments seen with hot acid extractions. The hot water/cold acid extraction procedure resulted in the reproducible recovery of many hypothalamic peptides, including several novel peptides.

Original languageEnglish (US)
Pages (from-to)4667-4676
Number of pages10
JournalJournal of Proteome Research
Volume6
Issue number12
DOIs
StatePublished - Dec 2007

Fingerprint

Neuropeptides
Hypothalamus
Peptides
Proteolysis
Acids
Sonication
Water
Centrifugation
Recovery
Degradation
Heating
Proteins

Keywords

  • Carboxypeptidase D
  • Carboxypeptidase E
  • Peptide processing
  • Prohormone convertase

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Optimization of neuropeptide extraction from the mouse hypothalamus. / Che, Fa Yun; Zhang, Xin; Berezniuk, Iryna; Callaway, Myrasol; Lim, Jihyeon; Fricker, Lloyd D.

In: Journal of Proteome Research, Vol. 6, No. 12, 12.2007, p. 4667-4676.

Research output: Contribution to journalArticle

Che, FY, Zhang, X, Berezniuk, I, Callaway, M, Lim, J & Fricker, LD 2007, 'Optimization of neuropeptide extraction from the mouse hypothalamus', Journal of Proteome Research, vol. 6, no. 12, pp. 4667-4676. https://doi.org/10.1021/pr060690r
Che, Fa Yun ; Zhang, Xin ; Berezniuk, Iryna ; Callaway, Myrasol ; Lim, Jihyeon ; Fricker, Lloyd D. / Optimization of neuropeptide extraction from the mouse hypothalamus. In: Journal of Proteome Research. 2007 ; Vol. 6, No. 12. pp. 4667-4676.
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