Optimization of medium constituents and fermentation conditions for the production of ethanol from palmyra jaggery using response surface methodology

B. V V Ratnam, S. Subba Rao, M. Damodar Rao, M. Narasimha Rao, C. Ayyanna

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The quantitative effects of sugar concentration, nitrogen concentration, EDTA, temperature, pH and time of fermentation on ethanol production were optimized using a Box-Wilson central composite design (CCD) experiment. It was found that palmyra jaggery (sugar syrup from the palmyra palm) is a suitable substrate for the production of high concentrations of ethanol using Saccharomyces cerevisiae NCIM 3090 by submerged fermentation. A maximum ethanol concentration of 129.4 g/l was obtained after optimizing media components and conditions of fermentation. The optimum values were a temperature of 26.2°C, pH of 8.4, time of fermentation of 4.2 days with 398.5 g of substrate/l, 3.1 g of urea/l and 0.51 g of EDTA/l. Thus by using the CCD, it is possible to determine the accurate values of the fermentation parameters where maximum production of ethanol occurs.

Original languageEnglish (US)
Pages (from-to)399-404
Number of pages6
JournalWorld Journal of Microbiology and Biotechnology
Volume21
Issue number4
DOIs
StatePublished - Jun 2005
Externally publishedYes

Fingerprint

jaggery
ethanol production
response surface methodology
Fermentation
Ethanol
fermentation
Borassus flabellifer
Edetic Acid
Sugars
ethanol
sugars
submerged fermentation
syrups
Temperature
Composite materials
Saccharomyces cerevisiae
Substrates
temperature
urea
experimental design

Keywords

  • Central composite design (CCD)
  • Ethanol
  • Palmyra jaggery
  • Response surface methodology (RSM)
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Plant Science
  • Biochemistry
  • Food Science
  • Microbiology
  • Biotechnology

Cite this

Optimization of medium constituents and fermentation conditions for the production of ethanol from palmyra jaggery using response surface methodology. / Ratnam, B. V V; Rao, S. Subba; Rao, M. Damodar; Rao, M. Narasimha; Ayyanna, C.

In: World Journal of Microbiology and Biotechnology, Vol. 21, No. 4, 06.2005, p. 399-404.

Research output: Contribution to journalArticle

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