Optimization of medium constituents and fermentation conditions for the production of ethanol from palmyra jaggery using response surface methodology

B. V.V. Ratnam, S. Subba Rao, M. Damodar Rao, M. Narasimha Rao, C. Ayyanna

Research output: Contribution to journalArticle

27 Scopus citations


The quantitative effects of sugar concentration, nitrogen concentration, EDTA, temperature, pH and time of fermentation on ethanol production were optimized using a Box-Wilson central composite design (CCD) experiment. It was found that palmyra jaggery (sugar syrup from the palmyra palm) is a suitable substrate for the production of high concentrations of ethanol using Saccharomyces cerevisiae NCIM 3090 by submerged fermentation. A maximum ethanol concentration of 129.4 g/l was obtained after optimizing media components and conditions of fermentation. The optimum values were a temperature of 26.2°C, pH of 8.4, time of fermentation of 4.2 days with 398.5 g of substrate/l, 3.1 g of urea/l and 0.51 g of EDTA/l. Thus by using the CCD, it is possible to determine the accurate values of the fermentation parameters where maximum production of ethanol occurs.

Original languageEnglish (US)
Pages (from-to)399-404
Number of pages6
JournalWorld Journal of Microbiology and Biotechnology
Issue number4
Publication statusPublished - Jun 1 2005



  • Central composite design (CCD)
  • Ethanol
  • Palmyra jaggery
  • Response surface methodology (RSM)
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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