Optical imaging in vitro provides evidence for the minicolumnar nature of cortical response

Adam Kohn, Aluisio Pinheiro, Mark A. Tommerdahl, Barry L. Whitsel

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The response of rat neocortical slices to electrical stimulation at the layer VI/white matter border was recorded using intrinsic signal optical imaging. The optical response of the slice is column-shaped, extends from layer VI to the pial surface, and is strongly correlated with the amplitude of simultaneously recorded evoked potentials. Spectral analysis revealed radially oriented spatial variations in the intensity of the optical signal with a period of 30-60 μm/cycle. Nissl-stained sections of slices also exhibited a radially oriented periodicity in optical density with the same period. We conclude that the periodic variations in the intrinsic optical signal correspond to stimulus-activated minicolumns.

Original languageEnglish (US)
Pages (from-to)3513-3518
Number of pages6
JournalNeuroReport
Volume8
Issue number16
Publication statusPublished - Dec 2 1997
Externally publishedYes

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Keywords

  • In vitro
  • Intrinsic optical signal
  • Minicolumns
  • Neocortex
  • Optical imaging

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kohn, A., Pinheiro, A., Tommerdahl, M. A., & Whitsel, B. L. (1997). Optical imaging in vitro provides evidence for the minicolumnar nature of cortical response. NeuroReport, 8(16), 3513-3518.