Opposite effects of β2-adrenoceptor gene deletion on insulin signaling in liver and skeletal muscle

E. Cipolletta, C. Del Giudice, G. Santulli, B. Trimarco, G. Iaccarino

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Background and aim β2-Adrenoceptors (β2-ARs) are G protein-coupled receptors (GPCRs) expressed in the major insulin target tissues. The interplay between β2-AR and insulin pathways is involved in the maintenance of glucose homeostasis. The aim of this study was to explore the consequences of β2-ARs deletion on insulin sensitivity and insulin signaling cascade in metabolically active tissues. Methods and results We evaluated glucose homeostasis in skeletal muscle and liver of β2-AR-null mice (β2-AR−/−) by performing in vivo (glucose tolerance test and insulin tolerance test) and ex vivo (glucose uptake and glycogen determination) experiments. β2-AR gene deletion is associated with hepatic insulin resistance and preserved skeletal muscle insulin sensitivity. Importantly, we demonstrate that hepatic β2-AR regulates insulin-induced AKT activation via Grb2-mediated SRC recruitment through a Gi-independent mechanism. Conclusions β-AR stimulation contributes to the development of early stages of insulin resistance progression in the liver. Our findings indicate that the cross-talk between β2-AR and insulin signaling represents a fundamental target towards the development of novel therapeutic approaches to treat type 2 diabetes and metabolic syndrome.

Original languageEnglish (US)
Pages (from-to)615-623
Number of pages9
JournalNutrition, Metabolism and Cardiovascular Diseases
Issue number7
StatePublished - Jul 2017
Externally publishedYes


  • AKT
  • Insulin resistance
  • SRC
  • aPKCζ
  • β adrenoceptors

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics
  • Cardiology and Cardiovascular Medicine


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