TY - JOUR
T1 - Open Sesame
T2 - Activating dormant replication origins in the mouse immunoglobulin heavy chain (Igh) locus
AU - Borowiec, James A.
AU - Schildkraut, Carl L.
N1 - Funding Information:
This work was supported by National Institutes of Health awards 5R01-GM045751 (CLS), 5R01-GM083185 (JAB), and 3R01-GM083185-S1 (JAB/CLS), and the Empire State Stem Cell Fund through NYS contract C024348 (CLS). We thank Julian Blow and Aloys Schepers for critical comments on the manuscript. We apologize to those authors whose work could not be cited due to space limitations.
PY - 2011/6
Y1 - 2011/6
N2 - Chromosomal DNA replication in mammals initiates from replication origins whose activity differs in accordance with cell type and differentiation state. In addition to origins that are active in unperturbed conditions, chromosomes also contain dormant origins that can become functional in response to certain genotoxic stress conditions. Improper regulation of origin usage can cause genomic instability leading to tumorigenesis. We review findings from recent single-molecule DNA fiber studies examining replication of the mouse immunoglobulin heavy chain (Igh) locus, in which origin activity over a 400. kb region is subject to dramatic developmental regulation. Possible models are discussed to explain such differential origin usage, particularly during replication stress conditions that can activate dormant origins.
AB - Chromosomal DNA replication in mammals initiates from replication origins whose activity differs in accordance with cell type and differentiation state. In addition to origins that are active in unperturbed conditions, chromosomes also contain dormant origins that can become functional in response to certain genotoxic stress conditions. Improper regulation of origin usage can cause genomic instability leading to tumorigenesis. We review findings from recent single-molecule DNA fiber studies examining replication of the mouse immunoglobulin heavy chain (Igh) locus, in which origin activity over a 400. kb region is subject to dramatic developmental regulation. Possible models are discussed to explain such differential origin usage, particularly during replication stress conditions that can activate dormant origins.
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U2 - 10.1016/j.ceb.2011.04.004
DO - 10.1016/j.ceb.2011.04.004
M3 - Review article
C2 - 21571518
AN - SCOPUS:79957808242
SN - 0955-0674
VL - 23
SP - 284
EP - 292
JO - Current Opinion in Cell Biology
JF - Current Opinion in Cell Biology
IS - 3
ER -