TY - JOUR
T1 - ON THE STATE OF CHROMOPHORE PROTONATION IN RHODOPSIN
T2 - IMPLICATION FOR PRIMARY PHOTOCHEMISTRY IN VISUAL PIGMENTS
AU - Narva, D.
AU - Callender, R. H.
PY - 1980/8
Y1 - 1980/8
N2 - Resonance Raman multicomponent spectra of bovine rhodopsin, isorhodopsin, and bathorhodopsin are obtained at low temperature. Application of the double beam, ‘pump‐probe’ technique allows an extraction of the rhodopsin and bathorhodopsin spectra in both protonated and deuterated media. Our results show that the Schiff bases of both rhodopsin and bathorhodopsin are fully protonated and the degree of protonation is unaffected by the rhodopsin‐bathorhodopsin transformation. Further, the data support the concept or cis‐trans isomerization as occurring in this transition. The effect of these results on various models for the primary photochemical event in vision is discussed.
AB - Resonance Raman multicomponent spectra of bovine rhodopsin, isorhodopsin, and bathorhodopsin are obtained at low temperature. Application of the double beam, ‘pump‐probe’ technique allows an extraction of the rhodopsin and bathorhodopsin spectra in both protonated and deuterated media. Our results show that the Schiff bases of both rhodopsin and bathorhodopsin are fully protonated and the degree of protonation is unaffected by the rhodopsin‐bathorhodopsin transformation. Further, the data support the concept or cis‐trans isomerization as occurring in this transition. The effect of these results on various models for the primary photochemical event in vision is discussed.
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U2 - 10.1111/j.1751-1097.1980.tb04021.x
DO - 10.1111/j.1751-1097.1980.tb04021.x
M3 - Article
C2 - 6254097
AN - SCOPUS:0019050753
SN - 0031-8655
VL - 32
SP - 273
EP - 276
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
IS - 2
ER -