ON THE STATE OF CHROMOPHORE PROTONATION IN RHODOPSIN: IMPLICATION FOR PRIMARY PHOTOCHEMISTRY IN VISUAL PIGMENTS

D. Narva, R. H. Callender

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Resonance Raman multicomponent spectra of bovine rhodopsin, isorhodopsin, and bathorhodopsin are obtained at low temperature. Application of the double beam, ‘pump‐probe’ technique allows an extraction of the rhodopsin and bathorhodopsin spectra in both protonated and deuterated media. Our results show that the Schiff bases of both rhodopsin and bathorhodopsin are fully protonated and the degree of protonation is unaffected by the rhodopsin‐bathorhodopsin transformation. Further, the data support the concept or cis‐trans isomerization as occurring in this transition. The effect of these results on various models for the primary photochemical event in vision is discussed.

Original languageEnglish (US)
Pages (from-to)273-276
Number of pages4
JournalPhotochemistry and Photobiology
Volume32
Issue number2
DOIs
StatePublished - Aug 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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