On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin

A. G. Doukas, A. Pande, T. Suzuki, Robert Callender, B. Honig, M. Ottolenghi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Continuous-flow resonance Raman experiments carried out in bacteriorhodopsin show that the exchange of a deutron on the Schiff base with a proton takes place in times shorter than 3 ms. Exchange mechanisms based on a base-catalyzed deprotonation followed by reprotonation of the Schiff base are excluded. A mechanism is suggested in which a water molecule interacts directly with the Schiff base deutron in a concerted exchange mechanism. It appears that in the dark, the binding site is more accessible to neutral water molecules than to charged protons.

Original languageEnglish (US)
Pages (from-to)275-279
Number of pages5
JournalBiophysical Journal
Volume33
Issue number2 I
DOIs
StatePublished - 1981
Externally publishedYes

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Bacteriorhodopsins
Schiff Bases
Deuterium
Hydrogen
Protons
Water
Binding Sites

ASJC Scopus subject areas

  • Biophysics

Cite this

On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin. / Doukas, A. G.; Pande, A.; Suzuki, T.; Callender, Robert; Honig, B.; Ottolenghi, M.

In: Biophysical Journal, Vol. 33, No. 2 I, 1981, p. 275-279.

Research output: Contribution to journalArticle

Doukas, AG, Pande, A, Suzuki, T, Callender, R, Honig, B & Ottolenghi, M 1981, 'On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin', Biophysical Journal, vol. 33, no. 2 I, pp. 275-279. https://doi.org/10.1016/S0006-3495(81)84889-8
Doukas, A. G. ; Pande, A. ; Suzuki, T. ; Callender, Robert ; Honig, B. ; Ottolenghi, M. / On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin. In: Biophysical Journal. 1981 ; Vol. 33, No. 2 I. pp. 275-279.
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