On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin

A. G. Doukas; A. Pande; T. Suzuki; R. H. Callender; B. Honig; M. Ottolenghi

doi:10.1016/S0006-3495(81)84889-8

On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin

A. G. Doukas, A. Pande, T. Suzuki, R. H. Callender, B. Honig, M. Ottolenghi

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Continuous-flow resonance Raman experiments carried out in bacteriorhodopsin show that the exchange of a deuteron on the Schiff base with a proton takes place in times shorter than 3 ms. Exchange mechanisms based on a base-catalyzed deprotonation followed by reprotonation of the Schiff base are excluded. A mechanism is suggested in which a water molecule interacts directly with the Schiff base deuteron in a concerted exchange mechanism. It appears that in the dark, the binding site is more accessible to neutral water molecules than to charged protons.

Original language	English (US)
Pages (from-to)	275-279
Number of pages	5
Journal	Biophysical journal
Volume	33
Issue number	2
DOIs	https://doi.org/10.1016/S0006-3495(81)84889-8
State	Published - 1981
Externally published	Yes

ASJC Scopus subject areas

Biophysics

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10.1016/S0006-3495(81)84889-8

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title = "On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin",

abstract = "Continuous-flow resonance Raman experiments carried out in bacteriorhodopsin show that the exchange of a deuteron on the Schiff base with a proton takes place in times shorter than 3 ms. Exchange mechanisms based on a base-catalyzed deprotonation followed by reprotonation of the Schiff base are excluded. A mechanism is suggested in which a water molecule interacts directly with the Schiff base deuteron in a concerted exchange mechanism. It appears that in the dark, the binding site is more accessible to neutral water molecules than to charged protons.",

author = "Doukas, {A. G.} and A. Pande and T. Suzuki and Callender, {R. H.} and B. Honig and M. Ottolenghi",

note = "Funding Information: This work was supported in part by the National Science Foundation (PCM79-02683), by the National Institutes of Health (EY03142), by a City University PSC-BHE Faculty Award at the City College, and by the National Science Foundation (PCM80-1093 1) at the University of Illinois.",

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T1 - On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin

AU - Doukas, A. G.

AU - Pande, A.

AU - Suzuki, T.

AU - Callender, R. H.

AU - Honig, B.

AU - Ottolenghi, M.

N1 - Funding Information: This work was supported in part by the National Science Foundation (PCM79-02683), by the National Institutes of Health (EY03142), by a City University PSC-BHE Faculty Award at the City College, and by the National Science Foundation (PCM80-1093 1) at the University of Illinois.

PY - 1981

Y1 - 1981

N2 - Continuous-flow resonance Raman experiments carried out in bacteriorhodopsin show that the exchange of a deuteron on the Schiff base with a proton takes place in times shorter than 3 ms. Exchange mechanisms based on a base-catalyzed deprotonation followed by reprotonation of the Schiff base are excluded. A mechanism is suggested in which a water molecule interacts directly with the Schiff base deuteron in a concerted exchange mechanism. It appears that in the dark, the binding site is more accessible to neutral water molecules than to charged protons.

AB - Continuous-flow resonance Raman experiments carried out in bacteriorhodopsin show that the exchange of a deuteron on the Schiff base with a proton takes place in times shorter than 3 ms. Exchange mechanisms based on a base-catalyzed deprotonation followed by reprotonation of the Schiff base are excluded. A mechanism is suggested in which a water molecule interacts directly with the Schiff base deuteron in a concerted exchange mechanism. It appears that in the dark, the binding site is more accessible to neutral water molecules than to charged protons.

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On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin

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