On the measurement of 15N-{1H} nuclear Overhauser effects. 2. Effects of the saturation scheme and water signal suppression

Fabien Ferrage, Amy Reichel, Shibani Battacharya, David Cowburn, Ranajeet Ghose

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Measurement of steady-state 15N-{1H} nuclear Overhauser effects forms a cornerstone of most methods to determine protein backbone dynamics from spin-relaxation data, since it is the most reliable probe of very fast motions on the ps-ns timescale. We have, in two previous publications (J. Magn. Reson. 192 (2008) 302-313; J. Am. Chem. Soc. 131 (2009) 6048-6049) reevaluated spin-dynamics during steady-state (or "saturated") and reference experiments, both of which are required to determine the NOE ratio. Here we assess the performance of several windowed and windowless sequences to achieve effective saturation of protons in steady-state experiments. We also evaluate the influence of the residual water signal due to radiation damping on the NOE ratio. We suggest a recipe that allows one to determine steady-state 15N-{1H} NOE's without artifacts and with the highest possible accuracy.

Original languageEnglish (US)
Pages (from-to)294-303
Number of pages10
JournalJournal of Magnetic Resonance
Issue number2
StatePublished - Dec 2010


  • Composite-pulse decoupling
  • Homogeneous master equation
  • Nuclear Overhauser effect
  • Protein dynamics
  • Radiation damping

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics


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