TY - JOUR
T1 - Omics Approaches for Understanding Biogenesis, Composition and Functions of Fungal Extracellular Vesicles
AU - Zamith-Miranda, Daniel
AU - Peres da Silva, Roberta
AU - Couvillion, Sneha P.
AU - Bredeweg, Erin L.
AU - Burnet, Meagan C.
AU - Coelho, Carolina
AU - Camacho, Emma
AU - Nimrichter, Leonardo
AU - Puccia, Rosana
AU - Almeida, Igor C.
AU - Casadevall, Arturo
AU - Rodrigues, Marcio L.
AU - Alves, Lysangela R.
AU - Nosanchuk, Joshua D.
AU - Nakayasu, Ernesto S.
N1 - Publisher Copyright:
© Copyright © 2021 Zamith-Miranda, Peres da Silva, Couvillion, Bredeweg, Burnet, Coelho, Camacho, Nimrichter, Puccia, Almeida, Casadevall, Rodrigues, Alves, Nosanchuk and Nakayasu.
PY - 2021/5/3
Y1 - 2021/5/3
N2 - Extracellular vesicles (EVs) are lipid bilayer structures released by organisms from all kingdoms of life. The diverse biogenesis pathways of EVs result in a wide variety of physical properties and functions across different organisms. Fungal EVs were first described in 2007 and different omics approaches have been fundamental to understand their composition, biogenesis, and function. In this review, we discuss the role of omics in elucidating fungal EVs biology. Transcriptomics, proteomics, metabolomics, and lipidomics have each enabled the molecular characterization of fungal EVs, providing evidence that these structures serve a wide array of functions, ranging from key carriers of cell wall biosynthetic machinery to virulence factors. Omics in combination with genetic approaches have been instrumental in determining both biogenesis and cargo loading into EVs. We also discuss how omics technologies are being employed to elucidate the role of EVs in antifungal resistance, disease biomarkers, and their potential use as vaccines. Finally, we review recent advances in analytical technology and multi-omic integration tools, which will help to address key knowledge gaps in EVs biology and translate basic research information into urgently needed clinical applications such as diagnostics, and immuno- and chemotherapies to fungal infections.
AB - Extracellular vesicles (EVs) are lipid bilayer structures released by organisms from all kingdoms of life. The diverse biogenesis pathways of EVs result in a wide variety of physical properties and functions across different organisms. Fungal EVs were first described in 2007 and different omics approaches have been fundamental to understand their composition, biogenesis, and function. In this review, we discuss the role of omics in elucidating fungal EVs biology. Transcriptomics, proteomics, metabolomics, and lipidomics have each enabled the molecular characterization of fungal EVs, providing evidence that these structures serve a wide array of functions, ranging from key carriers of cell wall biosynthetic machinery to virulence factors. Omics in combination with genetic approaches have been instrumental in determining both biogenesis and cargo loading into EVs. We also discuss how omics technologies are being employed to elucidate the role of EVs in antifungal resistance, disease biomarkers, and their potential use as vaccines. Finally, we review recent advances in analytical technology and multi-omic integration tools, which will help to address key knowledge gaps in EVs biology and translate basic research information into urgently needed clinical applications such as diagnostics, and immuno- and chemotherapies to fungal infections.
KW - extracellular vesicles
KW - fungi
KW - lipidomics
KW - metabolomics
KW - proteomics
KW - systems biology
KW - transcriptomics
KW - virulence
UR - http://www.scopus.com/inward/record.url?scp=85106017279&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85106017279&partnerID=8YFLogxK
U2 - 10.3389/fgene.2021.648524
DO - 10.3389/fgene.2021.648524
M3 - Review article
AN - SCOPUS:85106017279
SN - 1664-8021
VL - 12
JO - Frontiers in Genetics
JF - Frontiers in Genetics
M1 - 648524
ER -