Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system

O. S. Oliinyk; A. A. Kaberniuk; D. O. Burkaleva; S. I. Romaniuk; D. V. Kolibo; A. O. Shepelyakovskaya; A. G. Laman; S. V. Komisarenko

Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system

O. S. Oliinyk, A. A. Kaberniuk, D. O. Burkaleva, S. I. Romaniuk, D. V. Kolibo, A. O. Shepelyakovskaya, A. G. Laman, S. V. Komisarenko

Anatomy & Structural Biology

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4 Scopus citations

Abstract

Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 · 10⁷ M^-1 was selected. scFv preserved activity on storage in the course of 8 months.

Original language	English (US)
Pages (from-to)	91-97
Number of pages	7
Journal	Ukrain'skyi Biokhimichnyi Zhurnal
Volume	79
Issue number	5
Publication status	Published - Jan 1 2007

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Keywords

Diphtheria toxin
Phage display
Recombinant proteins
ScFv

ASJC Scopus subject areas

Biochemistry

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Oliinyk, O. S., Kaberniuk, A. A., Burkaleva, D. O., Romaniuk, S. I., Kolibo, D. V., Shepelyakovskaya, A. O., ... Komisarenko, S. V. (2007). Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system. Ukrain'skyi Biokhimichnyi Zhurnal, 79(5), 91-97.

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abstract = "Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 · 107 M-1 was selected. scFv preserved activity on storage in the course of 8 months.",

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AU - Burkaleva, D. O.

AU - Romaniuk, S. I.

AU - Kolibo, D. V.

AU - Shepelyakovskaya, A. O.

AU - Laman, A. G.

AU - Komisarenko, S. V.

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KW - Phage display

KW - Recombinant proteins

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Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system

Abstract

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Keywords

ASJC Scopus subject areas

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