Abstract
Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 · 107 M-1 was selected. scFv preserved activity on storage in the course of 8 months.
Original language | English (US) |
---|---|
Pages (from-to) | 91-97 |
Number of pages | 7 |
Journal | Ukrain'skyi Biokhimichnyi Zhurnal |
Volume | 79 |
Issue number | 5 |
State | Published - 2007 |
Externally published | Yes |
Keywords
- Diphtheria toxin
- Phage display
- Recombinant proteins
- ScFv
ASJC Scopus subject areas
- Biochemistry