Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system

O. S. Oliinyk; A. A. Kaberniuk; D. O. Burkaleva; S. I. Romaniuk; D. V. Kolibo; A. O. Shepelyakovskaya; A. G. Laman; S. V. Komisarenko

Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system

O. S. Oliinyk, A. A. Kaberniuk, D. O. Burkaleva, S. I. Romaniuk, D. V. Kolibo, A. O. Shepelyakovskaya, A. G. Laman, S. V. Komisarenko

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 · 10⁷ M^-1 was selected. scFv preserved activity on storage in the course of 8 months.

Original language	English (US)
Pages (from-to)	91-97
Number of pages	7
Journal	Ukrain'skyi Biokhimichnyi Zhurnal
Volume	79
Issue number	5
State	Published - 2007
Externally published	Yes

Keywords

Diphtheria toxin
Phage display
Recombinant proteins
ScFv

ASJC Scopus subject areas

Biochemistry

Cite this

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title = "Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system",

abstract = "Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 · 107 M-1 was selected. scFv preserved activity on storage in the course of 8 months.",

keywords = "Diphtheria toxin, Phage display, Recombinant proteins, ScFv",

author = "Oliinyk, {O. S.} and Kaberniuk, {A. A.} and Burkaleva, {D. O.} and Romaniuk, {S. I.} and Kolibo, {D. V.} and Shepelyakovskaya, {A. O.} and Laman, {A. G.} and Komisarenko, {S. V.}",

year = "2007",

language = "English (US)",

volume = "79",

pages = "91--97",

journal = "Ukrainski¿ biokhimicheski¿ zhurnal",

issn = "2409-4943",

publisher = "Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine (NASU)",

number = "5",

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T1 - Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system

AU - Oliinyk, O. S.

AU - Kaberniuk, A. A.

AU - Burkaleva, D. O.

AU - Romaniuk, S. I.

AU - Kolibo, D. V.

AU - Shepelyakovskaya, A. O.

AU - Laman, A. G.

AU - Komisarenko, S. V.

PY - 2007

Y1 - 2007

N2 - Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 · 107 M-1 was selected. scFv preserved activity on storage in the course of 8 months.

AB - Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 · 107 M-1 was selected. scFv preserved activity on storage in the course of 8 months.

KW - Diphtheria toxin

KW - Phage display

KW - Recombinant proteins

KW - ScFv

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ER -

Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system

Abstract

Keywords

ASJC Scopus subject areas

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