Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system

O. S. Oliinyk, A. A. Kaberniuk, D. O. Burkaleva, S. I. Romaniuk, D. V. Kolibo, A. O. Shepelyakovskaya, A. G. Laman, S. V. Komisarenko

Research output: Contribution to journal › Article

4 Scopus citations

Abstract

Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 · 107 M-1 was selected. scFv preserved activity on storage in the course of 8 months.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalUkrain'skyi Biokhimichnyi Zhurnal
Volume79
Issue number5
StatePublished - Jan 1 2007

Keywords

  • Diphtheria toxin
  • Phage display
  • Recombinant proteins
  • ScFv

ASJC Scopus subject areas

  • Biochemistry

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    Oliinyk, O. S., Kaberniuk, A. A., Burkaleva, D. O., Romaniuk, S. I., Kolibo, D. V., Shepelyakovskaya, A. O., Laman, A. G., & Komisarenko, S. V. (2007). Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system. Ukrain'skyi Biokhimichnyi Zhurnal, 79(5), 91-97.