Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system

O. S. Oliinyk; A. A. Kaberniuk; D. O. Burkaleva; S. I. Romaniuk; D. V. Kolibo; A. O. Shepelyakovskaya; A. G. Laman; S. V. Komisarenko

Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system

O. S. Oliinyk, A. A. Kaberniuk, D. O. Burkaleva, S. I. Romaniuk, D. V. Kolibo, A. O. Shepelyakovskaya, A. G. Laman, S. V. Komisarenko

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 · 10⁷ M^-1 was selected. scFv preserved activity on storage in the course of 8 months.

Original language	English (US)
Pages (from-to)	91-97
Number of pages	7
Journal	Ukrain'skyi Biokhimichnyi Zhurnal
Volume	79
Issue number	5
State	Published - 2007
Externally published	Yes

Keywords

Diphtheria toxin
Phage display
Recombinant proteins
ScFv

ASJC Scopus subject areas

Biochemistry

Cite this

Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system. / Oliinyk, O. S.; Kaberniuk, A. A.; Burkaleva, D. O.; Romaniuk, S. I.; Kolibo, D. V.; Shepelyakovskaya, A. O.; Laman, A. G.; Komisarenko, S. V.

In: Ukrain'skyi Biokhimichnyi Zhurnal, Vol. 79, No. 5, 2007, p. 91-97.

Research output: Contribution to journal › Article › peer-review

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abstract = "Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 · 107 M-1 was selected. scFv preserved activity on storage in the course of 8 months.",

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AU - Kaberniuk, A. A.

AU - Burkaleva, D. O.

AU - Romaniuk, S. I.

AU - Kolibo, D. V.

AU - Shepelyakovskaya, A. O.

AU - Laman, A. G.

AU - Komisarenko, S. V.

PY - 2007

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KW - Phage display

KW - Recombinant proteins

KW - ScFv

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Obtaining of recombinant scFv-antibodies against diphtheria toxin using phage display system

Abstract

Keywords

ASJC Scopus subject areas

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