Observation of the O-O stretching raman band for cytochrome P-450(cam) under catalytic conditions

T. Egawa, T. Ogura, R. Makino, Y. Ishimura, T. Kitagawa

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Dioxygen stretching (ν(oo)) Raman band was observed for the oxy form of Pseudomonas putida cytochrome P-450 (P-450(cam)) generated at room temperature under catalytic conditions, that is, in the presence of D-camphor, β-NADH, putidaredoxin, and putidaredoxin reductase, by using the mixed flow transient Raman apparatus. At the same time the visible absorption spectra were monitored for the transient species. It was found that the ν(oo) frequency is little altered by binding of putidaredoxin to P-450(cam), although the reduction rate of the oxy form becomes faster. Another intermediate with an oxygen isotope-sensitive band was not found in a time region until 2 s after mixing of the reduced enzyme with oxygen.

Original languageEnglish (US)
Pages (from-to)10246-10248
Number of pages3
JournalJournal of Biological Chemistry
Volume266
Issue number16
StatePublished - Aug 16 1991

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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