Observation of the O-O stretching raman band for cytochrome P-450cam under catalytic conditions

Tsuyoshi Egawa, Takashi Ogura, Ryu Makino, Yuzuru Ishimura, Teizo Kitagawa

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Dioxygen stretching (vOO) Raman band was observed for the oxy form of Pseudomonas putida cytochrome P-450 (P-450cam) generated at room temperature under catalytic conditions, that is, in the presence of Dcamphor, β-NADH, putidaredoxin, and putidaredoxin reductase, by using the mixed flow transient Raman apparatus. At the same time the visible absorption spectra were monitored for the transient species. It was found that the vOO frequency is little altered by binding of putidaredoxin to P-450cam, although the reduction rate of the oxy form becomes faster. Another intermediate with an oxygen isotope-sensitive band was not found in a time region until 2 s after mixing of the reduced enzyme with oxygen.

Original languageEnglish (US)
Pages (from-to)10246-10248
Number of pages3
JournalJournal of Biological Chemistry
Volume266
Issue number16
StatePublished - 1991
Externally publishedYes

Fingerprint

Cytochromes
Stretching
Oxygen Isotopes
Observation
Oxygen
Pseudomonas putida
NAD
Cytochrome P-450 Enzyme System
Absorption spectra
Temperature
Enzymes
putidaredoxin
putidaredoxin reductase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Egawa, T., Ogura, T., Makino, R., Ishimura, Y., & Kitagawa, T. (1991). Observation of the O-O stretching raman band for cytochrome P-450cam under catalytic conditions. Journal of Biological Chemistry, 266(16), 10246-10248.

Observation of the O-O stretching raman band for cytochrome P-450cam under catalytic conditions. / Egawa, Tsuyoshi; Ogura, Takashi; Makino, Ryu; Ishimura, Yuzuru; Kitagawa, Teizo.

In: Journal of Biological Chemistry, Vol. 266, No. 16, 1991, p. 10246-10248.

Research output: Contribution to journalArticle

Egawa, T, Ogura, T, Makino, R, Ishimura, Y & Kitagawa, T 1991, 'Observation of the O-O stretching raman band for cytochrome P-450cam under catalytic conditions', Journal of Biological Chemistry, vol. 266, no. 16, pp. 10246-10248.
Egawa, Tsuyoshi ; Ogura, Takashi ; Makino, Ryu ; Ishimura, Yuzuru ; Kitagawa, Teizo. / Observation of the O-O stretching raman band for cytochrome P-450cam under catalytic conditions. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 16. pp. 10246-10248.
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