O-fucosylation of muscle agrin determines its ability to cluster acetylcholine receptors

Mi Lyang Kim, Kumaran Chandrasekharan, Matthew Glass, Shaolin Shi, Mark C. Stahl, Brian Kaspar, Pamela Stanley, Paul T. Martin

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Protein O-fucosyltransferase 1 (Pofut1) transfers fucose to serine or threonine on proteins, including Notch receptors, that contain EGF repeats with a particular consensus sequence. Here we demonstrate that agrin is O-fucosylated in a Pofut1-dependent manner, and that this glycosylation can regulate agrin function. Fucosylation of recombinant C45 agrin, both active (neural, z8) and inactive (muscle, z0) splice forms, was eliminated when agrin was overexpressed in Pofut1-deficient cells or by mutation of a consensus site for Pofut1 fucosylation (serine 1726 in the EGF4 domain). Loss of O-fucosylation caused a gain of function for muscle agrin such that it stimulated AChR clustering and MuSK phosphorylation in cultured myotubes at levels normally only found with the neural splice form. Deletion of Pofut1 in cultured primary myotubes and in adult skeletal muscle increased AChR aggregation. In addition, Pofut1 gene and protein expression and Pofut1 activity of the EGF4 domain of agrin were modulated during neuromuscular development. These data are consistent with a role for Pofut1 in AChR aggregation during synaptogenesis via the regulation of the synaptogenic activity of muscle agrin.

Original languageEnglish (US)
Pages (from-to)452-464
Number of pages13
JournalMolecular and Cellular Neuroscience
Volume39
Issue number3
DOIs
StatePublished - Oct 29 2008

Fingerprint

Agrin
Cholinergic Receptors
Muscles
Proteins
Skeletal Muscle Fibers
Serine
Notch Receptors
Fucose
galactoside 2-alpha-L-fucosyltransferase
Consensus Sequence
Threonine
Glycosylation
Epidermal Growth Factor
Cluster Analysis
Skeletal Muscle

Keywords

  • Agrin
  • Fucose
  • Glycosylation
  • Neuromuscular junction
  • Notch
  • Synapse

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Kim, M. L., Chandrasekharan, K., Glass, M., Shi, S., Stahl, M. C., Kaspar, B., ... Martin, P. T. (2008). O-fucosylation of muscle agrin determines its ability to cluster acetylcholine receptors. Molecular and Cellular Neuroscience, 39(3), 452-464. https://doi.org/10.1016/j.mcn.2008.07.026

O-fucosylation of muscle agrin determines its ability to cluster acetylcholine receptors. / Kim, Mi Lyang; Chandrasekharan, Kumaran; Glass, Matthew; Shi, Shaolin; Stahl, Mark C.; Kaspar, Brian; Stanley, Pamela; Martin, Paul T.

In: Molecular and Cellular Neuroscience, Vol. 39, No. 3, 29.10.2008, p. 452-464.

Research output: Contribution to journalArticle

Kim, ML, Chandrasekharan, K, Glass, M, Shi, S, Stahl, MC, Kaspar, B, Stanley, P & Martin, PT 2008, 'O-fucosylation of muscle agrin determines its ability to cluster acetylcholine receptors', Molecular and Cellular Neuroscience, vol. 39, no. 3, pp. 452-464. https://doi.org/10.1016/j.mcn.2008.07.026
Kim, Mi Lyang ; Chandrasekharan, Kumaran ; Glass, Matthew ; Shi, Shaolin ; Stahl, Mark C. ; Kaspar, Brian ; Stanley, Pamela ; Martin, Paul T. / O-fucosylation of muscle agrin determines its ability to cluster acetylcholine receptors. In: Molecular and Cellular Neuroscience. 2008 ; Vol. 39, No. 3. pp. 452-464.
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