Nutritional requirements of papiliomavirus-transformed mouse cells and an unifected parent line in serum-free culture

Teresa P. Di Lorenzo, Joseph A. De Maro, Dorothy E. Pumo

Research output: Contribution to journalArticle

Abstract

A serum-free culture system was used to compare the nutritional requirements of mouse mammary cells transformed by bovine papillomavirus type 1 (ID13 cells) and the uninfected parent line (C127 cells). The serum-free, chemically defined medium used for this study was an MCDB 151-based medium (MCDB 151+S+I), supplemented with epidermal growth factor, transferrin, hydrocortisone, ethanolamine, phosphoethanolamine, retinoic acid, trace metals, and insulin. Proliferation of either cell type in serum-free culture required the addition of 250 μg/ml of insulin. ID13 cells have a doubling time of greater than 96 h in MCDB 151+S+I, whereas C127 cells have a doubling time of 60 h. This is in sharp contrast to the growth characteristics of the two cell types in 10% fetal bovine serum, where doubling times for the ID13 and C127 cells are 24 and 30 h, respectively. Culture of the cells in a serum-free medium has therefore revealed that the papillomavirus-transformed cells have more stringent growth requirements than the uninfected parent line.

Original languageEnglish (US)
Pages (from-to)909-913
Number of pages5
JournalIn Vitro Cellular & Developmental Biology
Volume25
Issue number10
DOIs
StatePublished - Oct 1 1989
Externally publishedYes

Keywords

  • C127
  • insulin
  • neuropeptides
  • papillomavirus
  • serum-free culture
  • transformation

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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