Nucleotide sequence and genomic structure analyses of the p70 subunit of the human Ku autoantigen: evidence for a family of genes encoding Ku (p70)-related polypeptides

Andrew J. Griffith, Joe Craft, Janine Evans, Tsuneyo Mimori, John A. Hardin

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

cDNA encoding the p70 polypeptide subunit of the human Ku autoantigen was isolated. In vitro expression analysis of the cDNA demonstrates that it encodes the entire open reading frame. Nucleotide sequence analysis and comparison to other previously described sequences indicate the existence of several single-nucleotide and amino acid polymorphisms. Southern blot analyses demonstrate the presence of multiple copies of homologous DNA sequences in the human genome. These data support the hypothesis that multiple genes encode a family of Ku(p70)-related polypeptides.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalMolecular Biology Reports
Volume16
Issue number2
DOIs
StatePublished - May 1 1992

Keywords

  • DNA-binding protein
  • Ku protein
  • autoantibodies
  • gene
  • recombinant DNA
  • systemic lupus erythematosus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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