Nucleotide sequence and genomic structure analyses of the p70 subunit of the human Ku autoantigen

evidence for a family of genes encoding Ku (p70)-related polypeptides

Andrew J. Griffith, Joe Craft, Janine Evans, Tsuneyo Mimori, John A. Hardin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

cDNA encoding the p70 polypeptide subunit of the human Ku autoantigen was isolated. In vitro expression analysis of the cDNA demonstrates that it encodes the entire open reading frame. Nucleotide sequence analysis and comparison to other previously described sequences indicate the existence of several single-nucleotide and amino acid polymorphisms. Southern blot analyses demonstrate the presence of multiple copies of homologous DNA sequences in the human genome. These data support the hypothesis that multiple genes encode a family of Ku(p70)-related polypeptides.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalMolecular Biology Reports
Volume16
Issue number2
DOIs
StatePublished - May 1992
Externally publishedYes

Fingerprint

Gene encoding
Autoantigens
Nucleotides
Complementary DNA
Genes
Peptides
DNA sequences
Human Genome
Sequence Homology
Southern Blotting
Polymorphism
Open Reading Frames
Sequence Analysis
Amino Acids
Ku Autoantigen
In Vitro Techniques

Keywords

  • autoantibodies
  • DNA-binding protein
  • gene
  • Ku protein
  • recombinant DNA
  • systemic lupus erythematosus

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Biochemistry

Cite this

Nucleotide sequence and genomic structure analyses of the p70 subunit of the human Ku autoantigen : evidence for a family of genes encoding Ku (p70)-related polypeptides. / Griffith, Andrew J.; Craft, Joe; Evans, Janine; Mimori, Tsuneyo; Hardin, John A.

In: Molecular Biology Reports, Vol. 16, No. 2, 05.1992, p. 91-97.

Research output: Contribution to journalArticle

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