Abstract
cDNA encoding the p70 polypeptide subunit of the human Ku autoantigen was isolated. In vitro expression analysis of the cDNA demonstrates that it encodes the entire open reading frame. Nucleotide sequence analysis and comparison to other previously described sequences indicate the existence of several single-nucleotide and amino acid polymorphisms. Southern blot analyses demonstrate the presence of multiple copies of homologous DNA sequences in the human genome. These data support the hypothesis that multiple genes encode a family of Ku(p70)-related polypeptides.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 91-97 |
| Number of pages | 7 |
| Journal | Molecular Biology Reports |
| Volume | 16 |
| Issue number | 2 |
| DOIs | |
| State | Published - May 1992 |
| Externally published | Yes |
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Keywords
- autoantibodies
- DNA-binding protein
- gene
- Ku protein
- recombinant DNA
- systemic lupus erythematosus
ASJC Scopus subject areas
- Genetics
- Cell Biology
- Biochemistry
Cite this
Nucleotide sequence and genomic structure analyses of the p70 subunit of the human Ku autoantigen : evidence for a family of genes encoding Ku (p70)-related polypeptides. / Griffith, Andrew J.; Craft, Joe; Evans, Janine; Mimori, Tsuneyo; Hardin, John A.
In: Molecular Biology Reports, Vol. 16, No. 2, 05.1992, p. 91-97.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nucleotide sequence and genomic structure analyses of the p70 subunit of the human Ku autoantigen
T2 - evidence for a family of genes encoding Ku (p70)-related polypeptides
AU - Griffith, Andrew J.
AU - Craft, Joe
AU - Evans, Janine
AU - Mimori, Tsuneyo
AU - Hardin, John A.
PY - 1992/5
Y1 - 1992/5
N2 - cDNA encoding the p70 polypeptide subunit of the human Ku autoantigen was isolated. In vitro expression analysis of the cDNA demonstrates that it encodes the entire open reading frame. Nucleotide sequence analysis and comparison to other previously described sequences indicate the existence of several single-nucleotide and amino acid polymorphisms. Southern blot analyses demonstrate the presence of multiple copies of homologous DNA sequences in the human genome. These data support the hypothesis that multiple genes encode a family of Ku(p70)-related polypeptides.
AB - cDNA encoding the p70 polypeptide subunit of the human Ku autoantigen was isolated. In vitro expression analysis of the cDNA demonstrates that it encodes the entire open reading frame. Nucleotide sequence analysis and comparison to other previously described sequences indicate the existence of several single-nucleotide and amino acid polymorphisms. Southern blot analyses demonstrate the presence of multiple copies of homologous DNA sequences in the human genome. These data support the hypothesis that multiple genes encode a family of Ku(p70)-related polypeptides.
KW - autoantibodies
KW - DNA-binding protein
KW - gene
KW - Ku protein
KW - recombinant DNA
KW - systemic lupus erythematosus
UR - http://www.scopus.com/inward/record.url?scp=0026862239&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026862239&partnerID=8YFLogxK
U2 - 10.1007/BF00419754
DO - 10.1007/BF00419754
M3 - Article
C2 - 1608402
AN - SCOPUS:0026862239
VL - 16
SP - 91
EP - 97
JO - Molecular Biology Reports
JF - Molecular Biology Reports
SN - 0301-4851
IS - 2
ER -