Abstract
cDNA encoding the p70 polypeptide subunit of the human Ku autoantigen was isolated. In vitro expression analysis of the cDNA demonstrates that it encodes the entire open reading frame. Nucleotide sequence analysis and comparison to other previously described sequences indicate the existence of several single-nucleotide and amino acid polymorphisms. Southern blot analyses demonstrate the presence of multiple copies of homologous DNA sequences in the human genome. These data support the hypothesis that multiple genes encode a family of Ku(p70)-related polypeptides.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 91-97 |
| Number of pages | 7 |
| Journal | Molecular Biology Reports |
| Volume | 16 |
| Issue number | 2 |
| DOIs | |
| State | Published - May 1 1992 |
Keywords
- DNA-binding protein
- Ku protein
- autoantibodies
- gene
- recombinant DNA
- systemic lupus erythematosus
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Fingerprint Dive into the research topics of 'Nucleotide sequence and genomic structure analyses of the p70 subunit of the human Ku autoantigen: evidence for a family of genes encoding Ku (p70)-related polypeptides'. Together they form a unique fingerprint.
Cite this
Griffith, A. J., Craft, J., Evans, J., Mimori, T., & Hardin, J. A. (1992). Nucleotide sequence and genomic structure analyses of the p70 subunit of the human Ku autoantigen: evidence for a family of genes encoding Ku (p70)-related polypeptides. Molecular Biology Reports, 16(2), 91-97. https://doi.org/10.1007/BF00419754