Nucleotide- and Activator-Dependent Structural and Dynamic Changes of Arp2/3 Complex Monitored by Hydrogen/Deuterium Exchange and Mass Spectrometry

Wendy D. Zencheck, Hui Xiao, Brad J. Nolen, Ruth Hogue Angeletti, Thomas D. Pollard, Steven C. Almo

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Arp2/3 complex plays a central role in the de novo nucleation of filamentous actin as branches on existing filaments. The complex must bind ATP, protein activators [e.g., Wiskott-Aldrich syndrome protein (WASp)], and the side of an actin filament to form a new actin filament. Amide hydrogen/deuterium exchange coupled with mass spectrometry was used to examine the structural and dynamic properties of the mammalian Arp2/3 complex in the presence of both ATP and the activating peptide segment from WASp. Changes in the rate of hydrogen exchange indicate that ATP binding causes conformational rearrangements of Arp2 and Arp3 that are transmitted allosterically to the Arp complex (ARPC)1, ARPC2, ARPC4, and ARPC5 subunits. These data are consistent with the closure of nucleotide-binding cleft of Arp3 upon ATP binding, resulting in structural rearrangements that propagate throughout the complex. Binding of the VCA domain of WASp to ATP-Arp2/3 further modulates the rates of hydrogen exchange in these subunits, indicating that a global conformational reorganization is occurring. These effects may include the direct binding of activators to Arp3, Arp2, and ARPC1; alterations in the relative orientations of Arp2 and Arp3; and the long-range transmission of activator-dependent signals to segments proposed to be involved in binding the F-actin mother filament.

Original languageEnglish (US)
Pages (from-to)414-427
Number of pages14
JournalJournal of Molecular Biology
Volume390
Issue number3
DOIs
StatePublished - Jul 17 2009

Fingerprint

Actin-Related Protein 2-3 Complex
Deuterium
Wiskott-Aldrich Syndrome Protein
Hydrogen
Mass Spectrometry
Nucleotides
Adenosine Triphosphate
Actin Cytoskeleton
Actins
Amides
Peptides
Proteins

Keywords

  • actin
  • amide hydrogen/deuterium exchange
  • Arp2/3
  • cytoskeleton
  • dynamics

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Nucleotide- and Activator-Dependent Structural and Dynamic Changes of Arp2/3 Complex Monitored by Hydrogen/Deuterium Exchange and Mass Spectrometry. / Zencheck, Wendy D.; Xiao, Hui; Nolen, Brad J.; Angeletti, Ruth Hogue; Pollard, Thomas D.; Almo, Steven C.

In: Journal of Molecular Biology, Vol. 390, No. 3, 17.07.2009, p. 414-427.

Research output: Contribution to journalArticle

Zencheck, Wendy D. ; Xiao, Hui ; Nolen, Brad J. ; Angeletti, Ruth Hogue ; Pollard, Thomas D. ; Almo, Steven C. / Nucleotide- and Activator-Dependent Structural and Dynamic Changes of Arp2/3 Complex Monitored by Hydrogen/Deuterium Exchange and Mass Spectrometry. In: Journal of Molecular Biology. 2009 ; Vol. 390, No. 3. pp. 414-427.
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