Nucleic acid and protein sequences of phosphocholine-binding light chains

S. P. Kwan, S. Rudikoff, J. G. Seidman, P. Leder, Matthew D. Scharff

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

An 18-kilobase DNA fragment containing the sequence coding for both the variable and constant regions of the S107 mouse immunoglobulin light chain was cloned from total cellular DNA. The complete nucleotide sequence of the κ-chain variable-region gene is reported. Determination of the amino acid sequence encoded by the DNA is found to be identical to the protein sequence of the T15 light chain through residue 88. Direct sequence analysis confirmed that the J1 joining segment is used in the recombination event producing the active κ light chain gene.

Original languageEnglish (US)
Pages (from-to)1366-1370
Number of pages5
JournalJournal of Experimental Medicine
Volume153
Issue number5
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

Phosphorylcholine
Nucleic Acids
Light
DNA
Immunoglobulin Light Chains
Proteins
Protein Sequence Analysis
Genetic Recombination
Genes
Sequence Analysis

ASJC Scopus subject areas

  • Immunology

Cite this

Nucleic acid and protein sequences of phosphocholine-binding light chains. / Kwan, S. P.; Rudikoff, S.; Seidman, J. G.; Leder, P.; Scharff, Matthew D.

In: Journal of Experimental Medicine, Vol. 153, No. 5, 1981, p. 1366-1370.

Research output: Contribution to journalArticle

Kwan, S. P. ; Rudikoff, S. ; Seidman, J. G. ; Leder, P. ; Scharff, Matthew D. / Nucleic acid and protein sequences of phosphocholine-binding light chains. In: Journal of Experimental Medicine. 1981 ; Vol. 153, No. 5. pp. 1366-1370.
@article{2912f3bad526484e8de8e2e667c0df9c,
title = "Nucleic acid and protein sequences of phosphocholine-binding light chains",
abstract = "An 18-kilobase DNA fragment containing the sequence coding for both the variable and constant regions of the S107 mouse immunoglobulin light chain was cloned from total cellular DNA. The complete nucleotide sequence of the κ-chain variable-region gene is reported. Determination of the amino acid sequence encoded by the DNA is found to be identical to the protein sequence of the T15 light chain through residue 88. Direct sequence analysis confirmed that the J1 joining segment is used in the recombination event producing the active κ light chain gene.",
author = "Kwan, {S. P.} and S. Rudikoff and Seidman, {J. G.} and P. Leder and Scharff, {Matthew D.}",
year = "1981",
doi = "10.1084/jem.153.5.1366",
language = "English (US)",
volume = "153",
pages = "1366--1370",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "5",

}

TY - JOUR

T1 - Nucleic acid and protein sequences of phosphocholine-binding light chains

AU - Kwan, S. P.

AU - Rudikoff, S.

AU - Seidman, J. G.

AU - Leder, P.

AU - Scharff, Matthew D.

PY - 1981

Y1 - 1981

N2 - An 18-kilobase DNA fragment containing the sequence coding for both the variable and constant regions of the S107 mouse immunoglobulin light chain was cloned from total cellular DNA. The complete nucleotide sequence of the κ-chain variable-region gene is reported. Determination of the amino acid sequence encoded by the DNA is found to be identical to the protein sequence of the T15 light chain through residue 88. Direct sequence analysis confirmed that the J1 joining segment is used in the recombination event producing the active κ light chain gene.

AB - An 18-kilobase DNA fragment containing the sequence coding for both the variable and constant regions of the S107 mouse immunoglobulin light chain was cloned from total cellular DNA. The complete nucleotide sequence of the κ-chain variable-region gene is reported. Determination of the amino acid sequence encoded by the DNA is found to be identical to the protein sequence of the T15 light chain through residue 88. Direct sequence analysis confirmed that the J1 joining segment is used in the recombination event producing the active κ light chain gene.

UR - http://www.scopus.com/inward/record.url?scp=0019498437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019498437&partnerID=8YFLogxK

U2 - 10.1084/jem.153.5.1366

DO - 10.1084/jem.153.5.1366

M3 - Article

C2 - 6788890

AN - SCOPUS:0019498437

VL - 153

SP - 1366

EP - 1370

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 5

ER -