Nucleic acid and protein sequences of phosphocholine-binding light chains

S. P. Kwan; S. Rudikoff; J. G. Seidman; P. Leder; M. D. Scharff

doi:10.1084/jem.153.5.1366

Nucleic acid and protein sequences of phosphocholine-binding light chains

S. P. Kwan, S. Rudikoff, J. G. Seidman, P. Leder, M. D. Scharff

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

An 18-kilobase DNA fragment containing the sequence coding for both the variable and constant regions of the S107 mouse immunoglobulin light chain was cloned from total cellular DNA. The complete nucleotide sequence of the κ-chain variable-region gene is reported. Determination of the amino acid sequence encoded by the DNA is found to be identical to the protein sequence of the T15 light chain through residue 88. Direct sequence analysis confirmed that the J1 joining segment is used in the recombination event producing the active κ light chain gene.

Original language	English (US)
Pages (from-to)	1366-1370
Number of pages	5
Journal	Journal of Experimental Medicine
Volume	153
Issue number	5
DOIs	https://doi.org/10.1084/jem.153.5.1366
State	Published - 1981
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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abstract = "An 18-kilobase DNA fragment containing the sequence coding for both the variable and constant regions of the S107 mouse immunoglobulin light chain was cloned from total cellular DNA. The complete nucleotide sequence of the κ-chain variable-region gene is reported. Determination of the amino acid sequence encoded by the DNA is found to be identical to the protein sequence of the T15 light chain through residue 88. Direct sequence analysis confirmed that the J1 joining segment is used in the recombination event producing the active κ light chain gene.",

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AU - Kwan, S. P.

AU - Rudikoff, S.

AU - Seidman, J. G.

AU - Leder, P.

AU - Scharff, M. D.

PY - 1981

Y1 - 1981

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AB - An 18-kilobase DNA fragment containing the sequence coding for both the variable and constant regions of the S107 mouse immunoglobulin light chain was cloned from total cellular DNA. The complete nucleotide sequence of the κ-chain variable-region gene is reported. Determination of the amino acid sequence encoded by the DNA is found to be identical to the protein sequence of the T15 light chain through residue 88. Direct sequence analysis confirmed that the J1 joining segment is used in the recombination event producing the active κ light chain gene.

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JF - Journal of Experimental Medicine

IS - 5

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Nucleic acid and protein sequences of phosphocholine-binding light chains

Abstract

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