Nuclear RNP complex assembly initiates cytoplasmic RNA localization

Tracy L. Kress, Young J. Yoon, Kimberly L. Mowry

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Cytoplasmic localization of mRNAs is a widespread mechanism for generating cell polarity and can provide the basis for patterning during embryonic development. A prominent example of this is localization of maternal mRNAs in Xenopus oocytes, a process requiring recognition of essential RNA sequences by protein components of the localization machinery. However, it is not yet clear how and when such protein factors associate with localized RNAs to carry out RNA transport. To trace the RNA-protein interactions that mediate RNA localization, we analyzed RNP complexes from the nucleus and cytoplasm. We find that an early step in the localization pathway is recognition of localized RNAs by specific RNA-binding proteins in the nucleus. After transport into the cytoplasm, the RNP complex is remodeled and additional transport factors are recruited. These results suggest that cytoplasmic RNA localization initiates in the nucleus and that binding of specific RNA-binding proteins in the nucleus may act to target RNAs to their appropriate destinations in the cytoplasm.

Original languageEnglish (US)
Pages (from-to)203-211
Number of pages9
JournalJournal of Cell Biology
Volume165
Issue number2
DOIs
StatePublished - Apr 26 2004
Externally publishedYes

Fingerprint

RNA
Cytoplasm
RNA-Binding Proteins
RNA Transport
Stored Messenger RNA
Cell Polarity
Proteins
Xenopus
Oocytes
Embryonic Development
Messenger RNA

Keywords

  • Oocyte
  • RNA-binding protein
  • VegT
  • Vg1
  • Xenopus

ASJC Scopus subject areas

  • Cell Biology

Cite this

Nuclear RNP complex assembly initiates cytoplasmic RNA localization. / Kress, Tracy L.; Yoon, Young J.; Mowry, Kimberly L.

In: Journal of Cell Biology, Vol. 165, No. 2, 26.04.2004, p. 203-211.

Research output: Contribution to journalArticle

Kress, Tracy L. ; Yoon, Young J. ; Mowry, Kimberly L. / Nuclear RNP complex assembly initiates cytoplasmic RNA localization. In: Journal of Cell Biology. 2004 ; Vol. 165, No. 2. pp. 203-211.
@article{2907e154ba744e348dec6a7c26e7aa3d,
title = "Nuclear RNP complex assembly initiates cytoplasmic RNA localization",
abstract = "Cytoplasmic localization of mRNAs is a widespread mechanism for generating cell polarity and can provide the basis for patterning during embryonic development. A prominent example of this is localization of maternal mRNAs in Xenopus oocytes, a process requiring recognition of essential RNA sequences by protein components of the localization machinery. However, it is not yet clear how and when such protein factors associate with localized RNAs to carry out RNA transport. To trace the RNA-protein interactions that mediate RNA localization, we analyzed RNP complexes from the nucleus and cytoplasm. We find that an early step in the localization pathway is recognition of localized RNAs by specific RNA-binding proteins in the nucleus. After transport into the cytoplasm, the RNP complex is remodeled and additional transport factors are recruited. These results suggest that cytoplasmic RNA localization initiates in the nucleus and that binding of specific RNA-binding proteins in the nucleus may act to target RNAs to their appropriate destinations in the cytoplasm.",
keywords = "Oocyte, RNA-binding protein, VegT, Vg1, Xenopus",
author = "Kress, {Tracy L.} and Yoon, {Young J.} and Mowry, {Kimberly L.}",
year = "2004",
month = "4",
day = "26",
doi = "10.1083/jcb.200309145",
language = "English (US)",
volume = "165",
pages = "203--211",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "2",

}

TY - JOUR

T1 - Nuclear RNP complex assembly initiates cytoplasmic RNA localization

AU - Kress, Tracy L.

AU - Yoon, Young J.

AU - Mowry, Kimberly L.

PY - 2004/4/26

Y1 - 2004/4/26

N2 - Cytoplasmic localization of mRNAs is a widespread mechanism for generating cell polarity and can provide the basis for patterning during embryonic development. A prominent example of this is localization of maternal mRNAs in Xenopus oocytes, a process requiring recognition of essential RNA sequences by protein components of the localization machinery. However, it is not yet clear how and when such protein factors associate with localized RNAs to carry out RNA transport. To trace the RNA-protein interactions that mediate RNA localization, we analyzed RNP complexes from the nucleus and cytoplasm. We find that an early step in the localization pathway is recognition of localized RNAs by specific RNA-binding proteins in the nucleus. After transport into the cytoplasm, the RNP complex is remodeled and additional transport factors are recruited. These results suggest that cytoplasmic RNA localization initiates in the nucleus and that binding of specific RNA-binding proteins in the nucleus may act to target RNAs to their appropriate destinations in the cytoplasm.

AB - Cytoplasmic localization of mRNAs is a widespread mechanism for generating cell polarity and can provide the basis for patterning during embryonic development. A prominent example of this is localization of maternal mRNAs in Xenopus oocytes, a process requiring recognition of essential RNA sequences by protein components of the localization machinery. However, it is not yet clear how and when such protein factors associate with localized RNAs to carry out RNA transport. To trace the RNA-protein interactions that mediate RNA localization, we analyzed RNP complexes from the nucleus and cytoplasm. We find that an early step in the localization pathway is recognition of localized RNAs by specific RNA-binding proteins in the nucleus. After transport into the cytoplasm, the RNP complex is remodeled and additional transport factors are recruited. These results suggest that cytoplasmic RNA localization initiates in the nucleus and that binding of specific RNA-binding proteins in the nucleus may act to target RNAs to their appropriate destinations in the cytoplasm.

KW - Oocyte

KW - RNA-binding protein

KW - VegT

KW - Vg1

KW - Xenopus

UR - http://www.scopus.com/inward/record.url?scp=2142827074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2142827074&partnerID=8YFLogxK

U2 - 10.1083/jcb.200309145

DO - 10.1083/jcb.200309145

M3 - Article

VL - 165

SP - 203

EP - 211

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 2

ER -