Nuclear localization of anti-Hu antibody is not associated with in vitro cytotoxicity

Adília Hormigo; Frank Lieberman

doi:10.1016/0165-5728(94)90011-6

Nuclear localization of anti-Hu antibody is not associated with in vitro cytotoxicity

Adília Hormigo, Frank Lieberman

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

IgG fractions of sera containing anti-Hu antibodies or control sera were influenced with Hu-positive and Hu-negative cell lines. Anti-Hu IgG specifically localized in the nucleus of Hu-positive cells. Anti-Hu-positive and control sera were incubated with Hu-positive cells and human complement or peripheral blood mononuclear cells. Hu antibody caused neither complement-mediated lysis nor augmented antibody-dependent cell-mediated cytotoxicity. Anti-Hu IgG did not affect proliferation of Hu-positive cells. Anti-Hu antibodies may not play a direct role in tissue injury in patients with paraneoplastic encephalomyeloneuropathy and anti-Hu antibodies.

Original language	English (US)
Pages (from-to)	205-212
Number of pages	8
Journal	Journal of Neuroimmunology
Volume	55
Issue number	2
DOIs	https://doi.org/10.1016/0165-5728(94)90011-6
State	Published - Dec 1994
Externally published	Yes

Keywords

Anti-Hu antibody
Antibody internalization
Cell growth
Cytotoxicity
Paraneoplastic encephalomyeloneuropathy

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/0165-5728(94)90011-6

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@article{c35e29d0e8644febb683e99e508a4d33,

title = "Nuclear localization of anti-Hu antibody is not associated with in vitro cytotoxicity",

abstract = "IgG fractions of sera containing anti-Hu antibodies or control sera were influenced with Hu-positive and Hu-negative cell lines. Anti-Hu IgG specifically localized in the nucleus of Hu-positive cells. Anti-Hu-positive and control sera were incubated with Hu-positive cells and human complement or peripheral blood mononuclear cells. Hu antibody caused neither complement-mediated lysis nor augmented antibody-dependent cell-mediated cytotoxicity. Anti-Hu IgG did not affect proliferation of Hu-positive cells. Anti-Hu antibodies may not play a direct role in tissue injury in patients with paraneoplastic encephalomyeloneuropathy and anti-Hu antibodies.",

keywords = "Anti-Hu antibody, Antibody internalization, Cell growth, Cytotoxicity, Paraneoplastic encephalomyeloneuropathy",

author = "Ad{\'i}lia Hormigo and Frank Lieberman",

note = "Funding Information: The authors would like to thank Dr. Paul Chapman for his contribution to the development of the cytotoxicity assays and Dr. Jerome B. Posner for his advice and comments both throughout this study and in the preparation of the manuscript. Dr. Josep Dalmau gracefully reviewed the manuscript. This work was supported in part by Funda~o Luso-Americana para o Desenvolvi-mento (A.H.)",

year = "1994",

month = dec,

doi = "10.1016/0165-5728(94)90011-6",

language = "English (US)",

volume = "55",

pages = "205--212",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Nuclear localization of anti-Hu antibody is not associated with in vitro cytotoxicity

AU - Hormigo, Adília

AU - Lieberman, Frank

N1 - Funding Information: The authors would like to thank Dr. Paul Chapman for his contribution to the development of the cytotoxicity assays and Dr. Jerome B. Posner for his advice and comments both throughout this study and in the preparation of the manuscript. Dr. Josep Dalmau gracefully reviewed the manuscript. This work was supported in part by Funda~o Luso-Americana para o Desenvolvi-mento (A.H.)

PY - 1994/12

Y1 - 1994/12

N2 - IgG fractions of sera containing anti-Hu antibodies or control sera were influenced with Hu-positive and Hu-negative cell lines. Anti-Hu IgG specifically localized in the nucleus of Hu-positive cells. Anti-Hu-positive and control sera were incubated with Hu-positive cells and human complement or peripheral blood mononuclear cells. Hu antibody caused neither complement-mediated lysis nor augmented antibody-dependent cell-mediated cytotoxicity. Anti-Hu IgG did not affect proliferation of Hu-positive cells. Anti-Hu antibodies may not play a direct role in tissue injury in patients with paraneoplastic encephalomyeloneuropathy and anti-Hu antibodies.

AB - IgG fractions of sera containing anti-Hu antibodies or control sera were influenced with Hu-positive and Hu-negative cell lines. Anti-Hu IgG specifically localized in the nucleus of Hu-positive cells. Anti-Hu-positive and control sera were incubated with Hu-positive cells and human complement or peripheral blood mononuclear cells. Hu antibody caused neither complement-mediated lysis nor augmented antibody-dependent cell-mediated cytotoxicity. Anti-Hu IgG did not affect proliferation of Hu-positive cells. Anti-Hu antibodies may not play a direct role in tissue injury in patients with paraneoplastic encephalomyeloneuropathy and anti-Hu antibodies.

KW - Anti-Hu antibody

KW - Antibody internalization

KW - Cell growth

KW - Cytotoxicity

KW - Paraneoplastic encephalomyeloneuropathy

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M3 - Article

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AN - SCOPUS:0028604443

SN - 0165-5728

VL - 55

SP - 205

EP - 212

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 2

ER -

Nuclear localization of anti-Hu antibody is not associated with in vitro cytotoxicity

Abstract

Keywords

ASJC Scopus subject areas

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