Novel transcribed regions in the human genome

J. Rozowsky, J. Wu, Z. Lian, U. Nagalakshmi, J. O. Korbel, P. Kapranov, Deyou Zheng, S. Dyke, P. Newburger, P. Miller, T. R. Gingeras, S. Weissman, M. Gerstein, M. Snyder

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have used genomic tiling arrays to identify transcribed regions throughout the human genome. Analysis of the mapping results of RNA isolated from five cell/tissue types, NB4 cells, NB4 cells treated with retinoic acid (RA), NB4 cells treated with 12-O-tetradecanoylphorbol-13 acetate (TPA), neutrophils, and placenta, throughout the ENCODE region reveals a large number of novel transcribed regions. Interestingly, neutrophils exhibit a great deal of novel expression in several intronic regions. Comparison of the hybridization results of NB4 cells treated with different stimuli relative to untreated cells reveals that many new regions are expressed upon cell differentiation. One such region is the Hox locus, which contains a large number of novel regions expressed in a number of cell types. Analysis of the trinucleotide composition of the novel transcribed regions reveals that it is similar to that of known exons. These results suggest that many of the novel transcribed regions may have a functional role. Copyright 2006, Cold Spring Harbor Laboratory Press

Original languageEnglish (US)
Pages (from-to)111-116
Number of pages6
JournalCold Spring Harbor Symposia on Quantitative Biology
Volume71
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Human Genome
Tretinoin
Exons
Acetates
Genes
RNA
Tissue
genome
Chemical analysis
cells
Neutrophils
neutrophils
Tetradecanoylphorbol Acetate
retinoic acid
Placenta
Cell Differentiation
placenta
cell differentiation
exons
Cell Count

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Biochemistry
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Rozowsky, J., Wu, J., Lian, Z., Nagalakshmi, U., Korbel, J. O., Kapranov, P., ... Snyder, M. (2006). Novel transcribed regions in the human genome. Cold Spring Harbor Symposia on Quantitative Biology, 71, 111-116. https://doi.org/10.1101/sqb.2006.71.054

Novel transcribed regions in the human genome. / Rozowsky, J.; Wu, J.; Lian, Z.; Nagalakshmi, U.; Korbel, J. O.; Kapranov, P.; Zheng, Deyou; Dyke, S.; Newburger, P.; Miller, P.; Gingeras, T. R.; Weissman, S.; Gerstein, M.; Snyder, M.

In: Cold Spring Harbor Symposia on Quantitative Biology, Vol. 71, 2006, p. 111-116.

Research output: Contribution to journalArticle

Rozowsky, J, Wu, J, Lian, Z, Nagalakshmi, U, Korbel, JO, Kapranov, P, Zheng, D, Dyke, S, Newburger, P, Miller, P, Gingeras, TR, Weissman, S, Gerstein, M & Snyder, M 2006, 'Novel transcribed regions in the human genome', Cold Spring Harbor Symposia on Quantitative Biology, vol. 71, pp. 111-116. https://doi.org/10.1101/sqb.2006.71.054
Rozowsky J, Wu J, Lian Z, Nagalakshmi U, Korbel JO, Kapranov P et al. Novel transcribed regions in the human genome. Cold Spring Harbor Symposia on Quantitative Biology. 2006;71:111-116. https://doi.org/10.1101/sqb.2006.71.054
Rozowsky, J. ; Wu, J. ; Lian, Z. ; Nagalakshmi, U. ; Korbel, J. O. ; Kapranov, P. ; Zheng, Deyou ; Dyke, S. ; Newburger, P. ; Miller, P. ; Gingeras, T. R. ; Weissman, S. ; Gerstein, M. ; Snyder, M. / Novel transcribed regions in the human genome. In: Cold Spring Harbor Symposia on Quantitative Biology. 2006 ; Vol. 71. pp. 111-116.
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