Novel Striatal GABAergic Interneuron Populations Labeled in the 5HT3aEGFP Mouse

A. B. Muñoz-Manchado, C. Foldi, S. Szydlowski, Lucas L. Sjulson, M. Farries, C. Wilson, G. Silberberg, J. Hjerling-Leffler

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Histological and morphological studies indicate that approximately 5% of striatal neurons are cholinergic or γ-aminobutyric acidergic (GABAergic) interneurons (gINs). However, the number of striatal neurons expressing known interneuron markers is too small to account for the entire interneuron population. We therefore studied the serotonin (5HT) receptor 3a-enhanced green fluorescent protein (5HT3aEGFP) mouse, in which we found that a large number of striatal gINs are labeled. Roughly 20% of 5HT3aEGFP-positive cells co-express parvalbumin and exhibit fast-spiking (FS) electrophysiological properties. However, the majority of labeled neurons do not overlap with known molecular interneuron markers. Intrinsic electrical properties reveal at least 2 distinct novel subtypes: a late-spiking (LS) neuropeptide-Y (NPY)-negative neurogliaform (NGF) interneuron, and a large heterogeneous population with several features resembling low-threshold-spiking (LTS) interneurons that do not express somatostatin, NPY, or neuronal nitric oxide synthase. Although the 5HT3aEGFP NGF and LTS-like interneurons have electrophysiological properties similar to previously described populations, they are pharmacologically distinct. In direct contrast to previously described NPY+ LTS and NGF cells, LTS-like 5HT3aEGFP cells show robust responses to nicotine administration, while the 5HT3aEGFP NGF cell type shows little or no response. By constructing a molecular map of the overlap between these novel populations and existing interneuron populations, we are able to reconcile the morphological and molecular estimates of striatal interneuron numbers.

Original languageEnglish (US)
Pages (from-to)96-105
Number of pages10
JournalCerebral Cortex
Volume26
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Corpus Striatum
Interneurons
Population
Neuropeptide Y
enhanced green fluorescent protein
Neurons
Parvalbumins
Nitric Oxide Synthase Type I
Cholinergic Neurons
Serotonin Receptors
Somatostatin
Nicotine

Keywords

  • 5HT3a
  • interneuron
  • nicotine
  • striatum
  • subtype

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

Muñoz-Manchado, A. B., Foldi, C., Szydlowski, S., Sjulson, L. L., Farries, M., Wilson, C., ... Hjerling-Leffler, J. (2016). Novel Striatal GABAergic Interneuron Populations Labeled in the 5HT3aEGFP Mouse. Cerebral Cortex, 26(1), 96-105. https://doi.org/10.1093/cercor/bhu179

Novel Striatal GABAergic Interneuron Populations Labeled in the 5HT3aEGFP Mouse. / Muñoz-Manchado, A. B.; Foldi, C.; Szydlowski, S.; Sjulson, Lucas L.; Farries, M.; Wilson, C.; Silberberg, G.; Hjerling-Leffler, J.

In: Cerebral Cortex, Vol. 26, No. 1, 01.01.2016, p. 96-105.

Research output: Contribution to journalArticle

Muñoz-Manchado, AB, Foldi, C, Szydlowski, S, Sjulson, LL, Farries, M, Wilson, C, Silberberg, G & Hjerling-Leffler, J 2016, 'Novel Striatal GABAergic Interneuron Populations Labeled in the 5HT3aEGFP Mouse', Cerebral Cortex, vol. 26, no. 1, pp. 96-105. https://doi.org/10.1093/cercor/bhu179
Muñoz-Manchado AB, Foldi C, Szydlowski S, Sjulson LL, Farries M, Wilson C et al. Novel Striatal GABAergic Interneuron Populations Labeled in the 5HT3aEGFP Mouse. Cerebral Cortex. 2016 Jan 1;26(1):96-105. https://doi.org/10.1093/cercor/bhu179
Muñoz-Manchado, A. B. ; Foldi, C. ; Szydlowski, S. ; Sjulson, Lucas L. ; Farries, M. ; Wilson, C. ; Silberberg, G. ; Hjerling-Leffler, J. / Novel Striatal GABAergic Interneuron Populations Labeled in the 5HT3aEGFP Mouse. In: Cerebral Cortex. 2016 ; Vol. 26, No. 1. pp. 96-105.
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