Abstract
We describe a novel two-dimensional liquid chromatography (2D-LC) method for fast and robust isolation and concentration of low abundant carbohydrates (sorbitol, glycerol) from biological matrices (plasma and urine). Off-line pre-purified fractions, enriched by analyte of interest, were analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS-MS). Initial 2D-LC automated sample pre-purification improved MS detection, eliminated matrix effects, and achieved high sensitivity (picogram detection limit) with a 6 min runtime and increased column lifetime. Using this method we have analyzed more than 1300 samples from biological matrices without column replacement.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11-16 |
| Number of pages | 6 |
| Journal | Journal of Chromatography A |
| Volume | 1073 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - May 6 2005 |
Fingerprint
Keywords
- 2D liquid chromatography
- Carbohydrate analysis
- Human plasma
- LC/MS
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Novel, highly robust method of carbohydrate pre-purification by two-dimensional liquid chromatography prior to liquid chromatography/mass spectrometry or gas chromatography/mass spectrometry. / Rogatsky, Eduard; Stein, Daniel T.
In: Journal of Chromatography A, Vol. 1073, No. 1-2, 06.05.2005, p. 11-16.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Novel, highly robust method of carbohydrate pre-purification by two-dimensional liquid chromatography prior to liquid chromatography/mass spectrometry or gas chromatography/mass spectrometry
AU - Rogatsky, Eduard
AU - Stein, Daniel T.
PY - 2005/5/6
Y1 - 2005/5/6
N2 - We describe a novel two-dimensional liquid chromatography (2D-LC) method for fast and robust isolation and concentration of low abundant carbohydrates (sorbitol, glycerol) from biological matrices (plasma and urine). Off-line pre-purified fractions, enriched by analyte of interest, were analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS-MS). Initial 2D-LC automated sample pre-purification improved MS detection, eliminated matrix effects, and achieved high sensitivity (picogram detection limit) with a 6 min runtime and increased column lifetime. Using this method we have analyzed more than 1300 samples from biological matrices without column replacement.
AB - We describe a novel two-dimensional liquid chromatography (2D-LC) method for fast and robust isolation and concentration of low abundant carbohydrates (sorbitol, glycerol) from biological matrices (plasma and urine). Off-line pre-purified fractions, enriched by analyte of interest, were analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS-MS). Initial 2D-LC automated sample pre-purification improved MS detection, eliminated matrix effects, and achieved high sensitivity (picogram detection limit) with a 6 min runtime and increased column lifetime. Using this method we have analyzed more than 1300 samples from biological matrices without column replacement.
KW - 2D liquid chromatography
KW - Carbohydrate analysis
KW - Human plasma
KW - LC/MS
UR - http://www.scopus.com/inward/record.url?scp=17444431263&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17444431263&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2005.03.074
DO - 10.1016/j.chroma.2005.03.074
M3 - Article
VL - 1073
SP - 11
EP - 16
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 1-2
ER -