Abstract
We describe a novel two-dimensional liquid chromatography (2D-LC) method for fast and robust isolation and concentration of low abundant carbohydrates (sorbitol, glycerol) from biological matrices (plasma and urine). Off-line pre-purified fractions, enriched by analyte of interest, were analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS-MS). Initial 2D-LC automated sample pre-purification improved MS detection, eliminated matrix effects, and achieved high sensitivity (picogram detection limit) with a 6 min runtime and increased column lifetime. Using this method we have analyzed more than 1300 samples from biological matrices without column replacement.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11-16 |
| Number of pages | 6 |
| Journal | Journal of Chromatography A |
| Volume | 1073 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - May 6 2005 |
Keywords
- 2D liquid chromatography
- Carbohydrate analysis
- Human plasma
- LC/MS
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
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Cite this
Rogatsky, E., & Stein, D. (2005). Novel, highly robust method of carbohydrate pre-purification by two-dimensional liquid chromatography prior to liquid chromatography/mass spectrometry or gas chromatography/mass spectrometry. Journal of Chromatography A, 1073(1-2), 11-16. https://doi.org/10.1016/j.chroma.2005.03.074