TY - JOUR
T1 - Novel, highly robust method of carbohydrate pre-purification by two-dimensional liquid chromatography prior to liquid chromatography/mass spectrometry or gas chromatography/mass spectrometry
AU - Rogatsky, Eduard
AU - Stein, Daniel
N1 - Funding Information:
These studies were supported by grants from the National Institutes of Health, 1 R01 DK61644-01 (DTS), MO1-RR12248, and the American Diabetes Association.
PY - 2005/5/6
Y1 - 2005/5/6
N2 - We describe a novel two-dimensional liquid chromatography (2D-LC) method for fast and robust isolation and concentration of low abundant carbohydrates (sorbitol, glycerol) from biological matrices (plasma and urine). Off-line pre-purified fractions, enriched by analyte of interest, were analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS-MS). Initial 2D-LC automated sample pre-purification improved MS detection, eliminated matrix effects, and achieved high sensitivity (picogram detection limit) with a 6 min runtime and increased column lifetime. Using this method we have analyzed more than 1300 samples from biological matrices without column replacement.
AB - We describe a novel two-dimensional liquid chromatography (2D-LC) method for fast and robust isolation and concentration of low abundant carbohydrates (sorbitol, glycerol) from biological matrices (plasma and urine). Off-line pre-purified fractions, enriched by analyte of interest, were analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS-MS). Initial 2D-LC automated sample pre-purification improved MS detection, eliminated matrix effects, and achieved high sensitivity (picogram detection limit) with a 6 min runtime and increased column lifetime. Using this method we have analyzed more than 1300 samples from biological matrices without column replacement.
KW - 2D liquid chromatography
KW - Carbohydrate analysis
KW - Human plasma
KW - LC/MS
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U2 - 10.1016/j.chroma.2005.03.074
DO - 10.1016/j.chroma.2005.03.074
M3 - Article
C2 - 15909500
AN - SCOPUS:17444431263
VL - 1073
SP - 11
EP - 16
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -