Novel genetic instability associated with a developmental regulated glycosyltransferase locus in Chinese hamster ovary cells

Sandra Sallustio, Pamela Stanley

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

LEC10 is a dominant glycosylation mutant of Chinese hamster ovary (CHO) cells that expresses a developmentally regulated glycosyltransferase (GlcNAc-TIII) not detectable in parental CHO cells. Several mutagens were found to increase the frequency of LEC10 mutants up to 10-fold over the spontaneous frequency of ≤10-7, while 5azaC treatment had no effect. Revenants were obtained at high frequency (∼10-4) and were found to belong to two classes. Three independent revertants gave rise to new LEC10 mutants at high frequency (∼10-4) while seven others gave new LEC10 mutants at the low frequency typical of unmutagenized parental CHO cells. No evidence of a general mutator phenotype was found in the revenant lines with a high rereversion frequency. The combined data suggest a novel form of genomic instability at the LEC10 locus in CHO cells. Genetic events that affect the expression of developmentally regulated glycosyltransferases may be identified by further studies of LEC10 and other dominant CHO glycosylation mutants.

Original languageEnglish (US)
Pages (from-to)387-400
Number of pages14
JournalSomatic Cell and Molecular Genetics
Volume15
Issue number5
DOIs
StatePublished - Sep 1989

Fingerprint

Glycosyltransferases
Cricetulus
Ovary
Glycosylation
Genomic Instability
Mutagens
Phenotype

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

@article{e0a49a2280b24ec991cc46ee2e169462,
title = "Novel genetic instability associated with a developmental regulated glycosyltransferase locus in Chinese hamster ovary cells",
abstract = "LEC10 is a dominant glycosylation mutant of Chinese hamster ovary (CHO) cells that expresses a developmentally regulated glycosyltransferase (GlcNAc-TIII) not detectable in parental CHO cells. Several mutagens were found to increase the frequency of LEC10 mutants up to 10-fold over the spontaneous frequency of ≤10-7, while 5azaC treatment had no effect. Revenants were obtained at high frequency (∼10-4) and were found to belong to two classes. Three independent revertants gave rise to new LEC10 mutants at high frequency (∼10-4) while seven others gave new LEC10 mutants at the low frequency typical of unmutagenized parental CHO cells. No evidence of a general mutator phenotype was found in the revenant lines with a high rereversion frequency. The combined data suggest a novel form of genomic instability at the LEC10 locus in CHO cells. Genetic events that affect the expression of developmentally regulated glycosyltransferases may be identified by further studies of LEC10 and other dominant CHO glycosylation mutants.",
author = "Sandra Sallustio and Pamela Stanley",
year = "1989",
month = "9",
doi = "10.1007/BF01534890",
language = "English (US)",
volume = "15",
pages = "387--400",
journal = "Somatic Cell and Molecular Genetics",
issn = "0740-7750",
publisher = "Springer GmbH & Co, Auslieferungs-Gesellschaf",
number = "5",

}

TY - JOUR

T1 - Novel genetic instability associated with a developmental regulated glycosyltransferase locus in Chinese hamster ovary cells

AU - Sallustio, Sandra

AU - Stanley, Pamela

PY - 1989/9

Y1 - 1989/9

N2 - LEC10 is a dominant glycosylation mutant of Chinese hamster ovary (CHO) cells that expresses a developmentally regulated glycosyltransferase (GlcNAc-TIII) not detectable in parental CHO cells. Several mutagens were found to increase the frequency of LEC10 mutants up to 10-fold over the spontaneous frequency of ≤10-7, while 5azaC treatment had no effect. Revenants were obtained at high frequency (∼10-4) and were found to belong to two classes. Three independent revertants gave rise to new LEC10 mutants at high frequency (∼10-4) while seven others gave new LEC10 mutants at the low frequency typical of unmutagenized parental CHO cells. No evidence of a general mutator phenotype was found in the revenant lines with a high rereversion frequency. The combined data suggest a novel form of genomic instability at the LEC10 locus in CHO cells. Genetic events that affect the expression of developmentally regulated glycosyltransferases may be identified by further studies of LEC10 and other dominant CHO glycosylation mutants.

AB - LEC10 is a dominant glycosylation mutant of Chinese hamster ovary (CHO) cells that expresses a developmentally regulated glycosyltransferase (GlcNAc-TIII) not detectable in parental CHO cells. Several mutagens were found to increase the frequency of LEC10 mutants up to 10-fold over the spontaneous frequency of ≤10-7, while 5azaC treatment had no effect. Revenants were obtained at high frequency (∼10-4) and were found to belong to two classes. Three independent revertants gave rise to new LEC10 mutants at high frequency (∼10-4) while seven others gave new LEC10 mutants at the low frequency typical of unmutagenized parental CHO cells. No evidence of a general mutator phenotype was found in the revenant lines with a high rereversion frequency. The combined data suggest a novel form of genomic instability at the LEC10 locus in CHO cells. Genetic events that affect the expression of developmentally regulated glycosyltransferases may be identified by further studies of LEC10 and other dominant CHO glycosylation mutants.

UR - http://www.scopus.com/inward/record.url?scp=0024420564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024420564&partnerID=8YFLogxK

U2 - 10.1007/BF01534890

DO - 10.1007/BF01534890

M3 - Article

VL - 15

SP - 387

EP - 400

JO - Somatic Cell and Molecular Genetics

JF - Somatic Cell and Molecular Genetics

SN - 0740-7750

IS - 5

ER -