Novel flavohemoglobins of mycobacteria

Sanjay Gupta, Sudesh Pawaria, Changyuan Lu, Syun-Ru Yeh, Kanak L. Dikshit

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Flavohemoglobins (flavoHbs) constitute a distinct class of chimeric hemoglobins in which a globin domain is coupled with a ferredoxin reductase such as FAD- and NADH-binding modules. Structural features and active site of heme and reductase domains are highly conserved in various flavoHbs. A new class of flavoHbs, displaying crucial differences in functionally conserved regions of heme and reductase domains, have been identified in mycobacteria. Mining of microbial genome data indicated that the occurrence of such flavoHbs might be restricted to a small group of microbes unlike conventional flavoHbs that are widespread among prokaryotes and lower eukaryotes. One of the representative flavoHbs of this class, encoded by Rv0385 gene (MtbFHb) of Mycobacterium tuberculosis, has been cloned, expressed, and characterized. The ferric and deoxy spectra of MtbFHb displayed a hexacoordinate state indicating that its distal site may be occupied by an intrinsic amino acid or an external ligand and it may not be involved in nitric oxide detoxification. Phylogenetic analysis revealed that mycobacterial flavoHbs constitute a separate cluster distinct from conventional flavoHbs and may have novel function(s).

Original languageEnglish (US)
Pages (from-to)337-345
Number of pages9
JournalIUBMB Life
Volume63
Issue number5
DOIs
StatePublished - May 2011

Fingerprint

Mycobacterium
Oxidoreductases
Heme
Genes
Microbial Genome
Ferredoxins
Detoxification
Flavin-Adenine Dinucleotide
Globins
Eukaryota
Mycobacterium tuberculosis
NAD
Catalytic Domain
Nitric Oxide
Hemoglobins
Ligands
Amino Acids

Keywords

  • electron-transfer
  • flavohemoglobin
  • mycobacteria
  • nitric-oxide dioxygenase
  • phylogeny

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Clinical Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Gupta, S., Pawaria, S., Lu, C., Yeh, S-R., & Dikshit, K. L. (2011). Novel flavohemoglobins of mycobacteria. IUBMB Life, 63(5), 337-345. https://doi.org/10.1002/iub.460

Novel flavohemoglobins of mycobacteria. / Gupta, Sanjay; Pawaria, Sudesh; Lu, Changyuan; Yeh, Syun-Ru; Dikshit, Kanak L.

In: IUBMB Life, Vol. 63, No. 5, 05.2011, p. 337-345.

Research output: Contribution to journalArticle

Gupta, S, Pawaria, S, Lu, C, Yeh, S-R & Dikshit, KL 2011, 'Novel flavohemoglobins of mycobacteria', IUBMB Life, vol. 63, no. 5, pp. 337-345. https://doi.org/10.1002/iub.460
Gupta S, Pawaria S, Lu C, Yeh S-R, Dikshit KL. Novel flavohemoglobins of mycobacteria. IUBMB Life. 2011 May;63(5):337-345. https://doi.org/10.1002/iub.460
Gupta, Sanjay ; Pawaria, Sudesh ; Lu, Changyuan ; Yeh, Syun-Ru ; Dikshit, Kanak L. / Novel flavohemoglobins of mycobacteria. In: IUBMB Life. 2011 ; Vol. 63, No. 5. pp. 337-345.
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