Novel dimeric interface and electrostatic recognition in bacterial Cu,Zn superoxide dismutase

Yves Bourne, Susan M. Redford, Howard M. Steinman, James R. Lepock, John A. Tainer, Elizabeth D. Getzoff

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Eukaryotic Cu,Zn superoxide dismutases (CuZnSODs) are antioxidant enzymes remarkable for their unusually stable β-barrel fold and dimer assembly, diffusion-limited catalysis, and electrostatic guidance of their free radical substrate. Point mutations of CuZnSOD cause the fatal human neurodegenerative disease amyotrophic lateral sclerosis. We determined and analyzed the first crystallographic structure (to our knowledge) for CuZnSOD from a prokaryote, Photobacterium leiognathi, a luminescent symbiont of Leiognathid fish. This structure, exemplifying prokaryotic CuZnSODs, shares the active-site ligand geometry and the topology of the Greek key β-barrel common to the eukaryotic CuZnSODs. However, the β-barrel elements recruited to form the dimer interface, the strategy used to forge the channel for electrostatic recognition of superoxide radical, and the connectivity of the intrasubunit disulfide bond in P. leiognathi CuZnSOD are discrete and strikingly dissimilar from those highly conserved in eukaryotic CuZnSODs. This new CuZnSOD structure broadens our understanding of structural features necessary and sufficient for CuZnSOD activity, highlights a hitherto unrecognized adaptability of the Greek key β-barrel building block in evolution, and reveals that prokaryotic and eukaryotic enzymes diverged from one primordial CuZnSOD and then converged to distinct dimeric enzymes with electrostatic substrate guidance.

Original languageEnglish (US)
Pages (from-to)12774-12779
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number23
DOIs
StatePublished - Nov 12 1996

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Static Electricity
Enzymes
Photobacterium
Superoxide Dismutase-1
Amyotrophic Lateral Sclerosis
Catalysis
Point Mutation
Superoxides
Disulfides
Neurodegenerative Diseases
Free Radicals
Catalytic Domain
Fishes
Antioxidants
Ligands

Keywords

  • β-barrel
  • evolution
  • macromolecular assembly
  • metalloenzyme
  • x-ray crystallography

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Novel dimeric interface and electrostatic recognition in bacterial Cu,Zn superoxide dismutase. / Bourne, Yves; Redford, Susan M.; Steinman, Howard M.; Lepock, James R.; Tainer, John A.; Getzoff, Elizabeth D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 23, 12.11.1996, p. 12774-12779.

Research output: Contribution to journalArticle

Bourne, Yves ; Redford, Susan M. ; Steinman, Howard M. ; Lepock, James R. ; Tainer, John A. ; Getzoff, Elizabeth D. / Novel dimeric interface and electrostatic recognition in bacterial Cu,Zn superoxide dismutase. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 23. pp. 12774-12779.
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