Novel cardiac protective effects of urea

From shark to rat

Xintao Wang, Wu Lingyun, M'hamed Aouffen, Mircea Alexandru Mateescu, Réginald Nadeau, Rui Wang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

1. This study was carried out to investigate novel cardioprotective effects of urea and the underlying mechanisms. The cardiac functions under oxidative stress were evaluated using Langendorff perfused isolated heart. 2. isolated dogfish shark hearts tolerated the oxidative stress generated by electrolysis (10 mA, 1 min) of the perfusion solution (n = 4), and also showed normal cardiac functions during post-ischaemia reperfusion (n = 4). The high concentration of urea (350 mM) in the heart perfusate was indispensable for maintaining the normal cardiac functions of the shark heart. 3. Urea at 3-300 mM (n = 4 for each group) protected the isolated rat heart against both electrolysis-induced heart damage and post-ischaemia reperfusion-induced cardiac injury. 4. A concentration-dependent scavenging effect of urea (3-300 mM, n = 4 for each group) against electrolysis-induced reactive oxygen species was also demonstrated in vitro. 5. Urea derivatives as hydroxyurea, dimethylurea, and thiourea had antioxidant cardioprotective effect against the electrolysis-induced cardiac dysfunction of rat heart, but were not as effective as urea in suppressing the post-ischaemia reperfusion injury. 6. Our results suggest that urea and its derivatives are potential antioxidant cardioprotective agents against oxidative stress-induced myocardium damage including the post-ischaemia reperfusion-induced injury.

Original languageEnglish (US)
Pages (from-to)1477-1484
Number of pages8
JournalBritish Journal of Pharmacology
Volume128
Issue number7
StatePublished - 1999
Externally publishedYes

Fingerprint

Sharks
Urea
Electrolysis
Oxidative Stress
Reperfusion Injury
Reperfusion
Ischemia
Antioxidants
Dogfish
Cardiotonic Agents
Thiourea
Hydroxyurea
Reactive Oxygen Species
Myocardium
Perfusion
Wounds and Injuries

Keywords

  • Arrhythmia
  • Heart
  • Ischaemia
  • Oxidative stress
  • Reperfusion
  • Urea

ASJC Scopus subject areas

  • Pharmacology

Cite this

Wang, X., Lingyun, W., Aouffen, M., Mateescu, M. A., Nadeau, R., & Wang, R. (1999). Novel cardiac protective effects of urea: From shark to rat. British Journal of Pharmacology, 128(7), 1477-1484.

Novel cardiac protective effects of urea : From shark to rat. / Wang, Xintao; Lingyun, Wu; Aouffen, M'hamed; Mateescu, Mircea Alexandru; Nadeau, Réginald; Wang, Rui.

In: British Journal of Pharmacology, Vol. 128, No. 7, 1999, p. 1477-1484.

Research output: Contribution to journalArticle

Wang, X, Lingyun, W, Aouffen, M, Mateescu, MA, Nadeau, R & Wang, R 1999, 'Novel cardiac protective effects of urea: From shark to rat', British Journal of Pharmacology, vol. 128, no. 7, pp. 1477-1484.
Wang X, Lingyun W, Aouffen M, Mateescu MA, Nadeau R, Wang R. Novel cardiac protective effects of urea: From shark to rat. British Journal of Pharmacology. 1999;128(7):1477-1484.
Wang, Xintao ; Lingyun, Wu ; Aouffen, M'hamed ; Mateescu, Mircea Alexandru ; Nadeau, Réginald ; Wang, Rui. / Novel cardiac protective effects of urea : From shark to rat. In: British Journal of Pharmacology. 1999 ; Vol. 128, No. 7. pp. 1477-1484.
@article{4e32ae1c51a5427784bb14ab976ff26a,
title = "Novel cardiac protective effects of urea: From shark to rat",
abstract = "1. This study was carried out to investigate novel cardioprotective effects of urea and the underlying mechanisms. The cardiac functions under oxidative stress were evaluated using Langendorff perfused isolated heart. 2. isolated dogfish shark hearts tolerated the oxidative stress generated by electrolysis (10 mA, 1 min) of the perfusion solution (n = 4), and also showed normal cardiac functions during post-ischaemia reperfusion (n = 4). The high concentration of urea (350 mM) in the heart perfusate was indispensable for maintaining the normal cardiac functions of the shark heart. 3. Urea at 3-300 mM (n = 4 for each group) protected the isolated rat heart against both electrolysis-induced heart damage and post-ischaemia reperfusion-induced cardiac injury. 4. A concentration-dependent scavenging effect of urea (3-300 mM, n = 4 for each group) against electrolysis-induced reactive oxygen species was also demonstrated in vitro. 5. Urea derivatives as hydroxyurea, dimethylurea, and thiourea had antioxidant cardioprotective effect against the electrolysis-induced cardiac dysfunction of rat heart, but were not as effective as urea in suppressing the post-ischaemia reperfusion injury. 6. Our results suggest that urea and its derivatives are potential antioxidant cardioprotective agents against oxidative stress-induced myocardium damage including the post-ischaemia reperfusion-induced injury.",
keywords = "Arrhythmia, Heart, Ischaemia, Oxidative stress, Reperfusion, Urea",
author = "Xintao Wang and Wu Lingyun and M'hamed Aouffen and Mateescu, {Mircea Alexandru} and R{\'e}ginald Nadeau and Rui Wang",
year = "1999",
language = "English (US)",
volume = "128",
pages = "1477--1484",
journal = "British Journal of Pharmacology",
issn = "0007-1188",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Novel cardiac protective effects of urea

T2 - From shark to rat

AU - Wang, Xintao

AU - Lingyun, Wu

AU - Aouffen, M'hamed

AU - Mateescu, Mircea Alexandru

AU - Nadeau, Réginald

AU - Wang, Rui

PY - 1999

Y1 - 1999

N2 - 1. This study was carried out to investigate novel cardioprotective effects of urea and the underlying mechanisms. The cardiac functions under oxidative stress were evaluated using Langendorff perfused isolated heart. 2. isolated dogfish shark hearts tolerated the oxidative stress generated by electrolysis (10 mA, 1 min) of the perfusion solution (n = 4), and also showed normal cardiac functions during post-ischaemia reperfusion (n = 4). The high concentration of urea (350 mM) in the heart perfusate was indispensable for maintaining the normal cardiac functions of the shark heart. 3. Urea at 3-300 mM (n = 4 for each group) protected the isolated rat heart against both electrolysis-induced heart damage and post-ischaemia reperfusion-induced cardiac injury. 4. A concentration-dependent scavenging effect of urea (3-300 mM, n = 4 for each group) against electrolysis-induced reactive oxygen species was also demonstrated in vitro. 5. Urea derivatives as hydroxyurea, dimethylurea, and thiourea had antioxidant cardioprotective effect against the electrolysis-induced cardiac dysfunction of rat heart, but were not as effective as urea in suppressing the post-ischaemia reperfusion injury. 6. Our results suggest that urea and its derivatives are potential antioxidant cardioprotective agents against oxidative stress-induced myocardium damage including the post-ischaemia reperfusion-induced injury.

AB - 1. This study was carried out to investigate novel cardioprotective effects of urea and the underlying mechanisms. The cardiac functions under oxidative stress were evaluated using Langendorff perfused isolated heart. 2. isolated dogfish shark hearts tolerated the oxidative stress generated by electrolysis (10 mA, 1 min) of the perfusion solution (n = 4), and also showed normal cardiac functions during post-ischaemia reperfusion (n = 4). The high concentration of urea (350 mM) in the heart perfusate was indispensable for maintaining the normal cardiac functions of the shark heart. 3. Urea at 3-300 mM (n = 4 for each group) protected the isolated rat heart against both electrolysis-induced heart damage and post-ischaemia reperfusion-induced cardiac injury. 4. A concentration-dependent scavenging effect of urea (3-300 mM, n = 4 for each group) against electrolysis-induced reactive oxygen species was also demonstrated in vitro. 5. Urea derivatives as hydroxyurea, dimethylurea, and thiourea had antioxidant cardioprotective effect against the electrolysis-induced cardiac dysfunction of rat heart, but were not as effective as urea in suppressing the post-ischaemia reperfusion injury. 6. Our results suggest that urea and its derivatives are potential antioxidant cardioprotective agents against oxidative stress-induced myocardium damage including the post-ischaemia reperfusion-induced injury.

KW - Arrhythmia

KW - Heart

KW - Ischaemia

KW - Oxidative stress

KW - Reperfusion

KW - Urea

UR - http://www.scopus.com/inward/record.url?scp=0032723586&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032723586&partnerID=8YFLogxK

M3 - Article

VL - 128

SP - 1477

EP - 1484

JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

SN - 0007-1188

IS - 7

ER -