Normal saline induces oxidative stress in peritoneal mesothelial cells

Alicja Połubinska, Andrzej Breborowicz, Ryszard Staniszewski, Dimitrios G. Oreopoulos

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Peritoneal adhesions are the most common complication of the abdominal surgery. Normal saline is frequently used to rinse the peritoneal cavity during abdominal surgery, although there is no well-established data describing effect of such procedure on the process of formation of peritoneal adhesions. Methods: Effect of 0.9% NaCl solution on viability, oxidative stress, and fibrinolytic activity of human peritoneal mesothelial cells maintained in in vitro culture was evaluated. Results: Exposure of mesothelial cells to 0.9% NaCl induces oxidative stress, derangement of their structure with subsequent increased release of tissue factor (+75%) and plasminogen activator inhibitor-1 (+19%), and simultaneous suppression of tissue plasminogen activator release (-39%). In effect, ration tissue plasminogen activator/plasminogen activator inhibitor-1 was reduced in 0.9% NaCl-treated cells by 50%. Pretreatment of cells with precursor of glutathione synthesis: l-2-oxothiazolidine-4-carboxylic acid prevented these changes. Conclusions: Oxidative stress in the peritoneal mesothelium caused by 0.9% NaCl activates their procoagulant activity and impairs fibrinolytic properties of these cells. These effects disqualify 0.9% NaCl as rinsing solution during abdominal surgery.

Original languageEnglish (US)
Pages (from-to)1821-1826
Number of pages6
JournalJournal of Pediatric Surgery
Volume43
Issue number10
DOIs
StatePublished - Oct 2008
Externally publishedYes

Fingerprint

Oxidative Stress
Plasminogen Activator Inhibitor 1
Tissue Plasminogen Activator
Peritoneal Cavity
Thromboplastin
Human Activities
Glutathione
Epithelium

Keywords

  • Adhesions
  • Fibrinolytic activity
  • Mesothelium
  • Normal saline
  • Oxidative stress

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

Cite this

Połubinska, A., Breborowicz, A., Staniszewski, R., & Oreopoulos, D. G. (2008). Normal saline induces oxidative stress in peritoneal mesothelial cells. Journal of Pediatric Surgery, 43(10), 1821-1826. https://doi.org/10.1016/j.jpedsurg.2008.02.010

Normal saline induces oxidative stress in peritoneal mesothelial cells. / Połubinska, Alicja; Breborowicz, Andrzej; Staniszewski, Ryszard; Oreopoulos, Dimitrios G.

In: Journal of Pediatric Surgery, Vol. 43, No. 10, 10.2008, p. 1821-1826.

Research output: Contribution to journalArticle

Połubinska, A, Breborowicz, A, Staniszewski, R & Oreopoulos, DG 2008, 'Normal saline induces oxidative stress in peritoneal mesothelial cells', Journal of Pediatric Surgery, vol. 43, no. 10, pp. 1821-1826. https://doi.org/10.1016/j.jpedsurg.2008.02.010
Połubinska, Alicja ; Breborowicz, Andrzej ; Staniszewski, Ryszard ; Oreopoulos, Dimitrios G. / Normal saline induces oxidative stress in peritoneal mesothelial cells. In: Journal of Pediatric Surgery. 2008 ; Vol. 43, No. 10. pp. 1821-1826.
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