Nonprotein based enrichment method to analyze peptide cross-linking in protein complexes

Funing Yan, Fa Yun Che, Dmitry Rykunov, Edward Nieves, Andras Fiser, Louis M. Weiss, Ruth Hogue Angeletti

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Cross-linking analysis of protein complexes and structures by tandem mass spectrometry (MS/MS) has advantages in speed, sensitivity, specificity, and the capability of handling complicated protein assemblies. However, detection and accurate assignment of the cross-linked peptides are often challenging due to their low abundance and complicated fragmentation behavior in collision-induced dissociation (CID). To simplify the MS analysis and improve the signal-to-noise ratio of the cross-linked peptides, we developed a novel peptide enrichment strategy that utilizes a cross-linker with a cryptic thiol group and using beads modified with a photocleavable cross-linker. The functional cross-linkers were designed to react with the primary amino groups in proteins. Human serum albumin was used as a model protein to detect intra- and intermolecular cross-linkages. Use of this protein-free selective retrieval method eliminates the contamination that can result from avidin - biotin based retrieval systems and simplifies data analysis. These features may make the method suitable to investigate protein - protein interactions in biological samples.

Original languageEnglish (US)
Pages (from-to)7149-7159
Number of pages11
JournalAnalytical Chemistry
Volume81
Issue number17
DOIs
StatePublished - Sep 1 2009

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Peptides
Proteins
Avidin
Biotin
Sulfhydryl Compounds
Serum Albumin
Mass spectrometry
Signal to noise ratio
Contamination

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Nonprotein based enrichment method to analyze peptide cross-linking in protein complexes. / Yan, Funing; Che, Fa Yun; Rykunov, Dmitry; Nieves, Edward; Fiser, Andras; Weiss, Louis M.; Angeletti, Ruth Hogue.

In: Analytical Chemistry, Vol. 81, No. 17, 01.09.2009, p. 7149-7159.

Research output: Contribution to journalArticle

Yan, Funing ; Che, Fa Yun ; Rykunov, Dmitry ; Nieves, Edward ; Fiser, Andras ; Weiss, Louis M. ; Angeletti, Ruth Hogue. / Nonprotein based enrichment method to analyze peptide cross-linking in protein complexes. In: Analytical Chemistry. 2009 ; Vol. 81, No. 17. pp. 7149-7159.
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