Nonperfusion of retina and choroid in transgenic mouse models of sickle cell disease

G. A. Lutty, C. Merges, D. S. McLeod, S. D. Wajer, S. M. Suzuka, M. E. Fabry, R. L. Nagel

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose. To determine if vascular occlusion and nonperfusion is associated with the outer retinal atrophy, retinopathy, and choroidopathy (chorioretinopathy) that occurs in the α(H)β(S)[β(MDV)] and α(H)β(S)[α(MD)β(MDD)] transgenic mouse models of sickle cell disease. Methods. Mice from the α(H)β(S)[β(MDD)] and α(H)β(S)[α(MD)β(MDD)] transgenic mouse lines that express high levels of human β5 globin were anesthetized and administered horseradish peroxidase (HRP) intracardially. After 1 min, the animals were sacrificed, and the retina from one eye was excised, fixed, and developed in diaminobenzidine (DAB). The contralateral eye was fixed, embedded whole in glycol methacrylate, and HRP developed in 2.5 μm sections. Results. HRP reaction product (HRP-RP) and stained erythrocytes (RBCs) (due to endogenous peroxidase) were diffusely distributed within all vascular lumens in flatmount retinas from control animals (littermates homozygous for the mouse β(Major) deletion not expressing the β5 transgene). In 42.5% of the transgenic mice expressing β5 without any proliferative retinopathy, many blood vessels contained RBC plugs and lacked lumenal HRP-RP. In addition to packed RBCs, fibrin was sometimes present at sites of occlusion. In sections from whole eyes of the same animals, foci of photoreceptor degeneration were associated with areas of choriocapillaris nonperfusion (lumen that lacked HRP-PR). In areas with normal photoreceptors, the choriocapillaris appeared perfused (HRP-RP was present). In animals with proliferative chorioretinopathy, some neovascular formations lacked luminal HRP-RP, suggesting autoinfarction. Conclusions. Nonperfused retinal and choroidal vessels were observed in mice from the α(H)β(S)[β(MDD)] and (α(H)β(S)[α(MD)β(MDD)] lines without retinal and choroidal neovascularization. whereas, all mice with neovascularization had nonperfused areas. Furthermore, small foci of PR loss were associated with areas of nonperfused choriocapillaris. These results suggest that sickle cell-mediated vaso-occlusions are an initial event in the chorioretinopathy and outer retinal atrophy that occurs in these models.

Original languageEnglish (US)
Pages (from-to)438-444
Number of pages7
JournalCurrent Eye Research
Volume17
Issue number4
StatePublished - Apr 1998

Fingerprint

Choroid
Sickle Cell Anemia
Horseradish Peroxidase
Transgenic Mice
Retina
Blood Vessels
Atrophy
Retinal Neovascularization
Retinal Vessels
Choroidal Neovascularization
Globins
Fibrin
Transgenes
Peroxidase
Erythrocytes

Keywords

  • Choroidal neovascularization
  • Horseradish peroxidase
  • Sickle cell disease
  • Transgenic mice
  • Vaso-occlusion

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Lutty, G. A., Merges, C., McLeod, D. S., Wajer, S. D., Suzuka, S. M., Fabry, M. E., & Nagel, R. L. (1998). Nonperfusion of retina and choroid in transgenic mouse models of sickle cell disease. Current Eye Research, 17(4), 438-444.

Nonperfusion of retina and choroid in transgenic mouse models of sickle cell disease. / Lutty, G. A.; Merges, C.; McLeod, D. S.; Wajer, S. D.; Suzuka, S. M.; Fabry, M. E.; Nagel, R. L.

In: Current Eye Research, Vol. 17, No. 4, 04.1998, p. 438-444.

Research output: Contribution to journalArticle

Lutty, GA, Merges, C, McLeod, DS, Wajer, SD, Suzuka, SM, Fabry, ME & Nagel, RL 1998, 'Nonperfusion of retina and choroid in transgenic mouse models of sickle cell disease', Current Eye Research, vol. 17, no. 4, pp. 438-444.
Lutty GA, Merges C, McLeod DS, Wajer SD, Suzuka SM, Fabry ME et al. Nonperfusion of retina and choroid in transgenic mouse models of sickle cell disease. Current Eye Research. 1998 Apr;17(4):438-444.
Lutty, G. A. ; Merges, C. ; McLeod, D. S. ; Wajer, S. D. ; Suzuka, S. M. ; Fabry, M. E. ; Nagel, R. L. / Nonperfusion of retina and choroid in transgenic mouse models of sickle cell disease. In: Current Eye Research. 1998 ; Vol. 17, No. 4. pp. 438-444.
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abstract = "Purpose. To determine if vascular occlusion and nonperfusion is associated with the outer retinal atrophy, retinopathy, and choroidopathy (chorioretinopathy) that occurs in the α(H)β(S)[β(MDV)] and α(H)β(S)[α(MD)β(MDD)] transgenic mouse models of sickle cell disease. Methods. Mice from the α(H)β(S)[β(MDD)] and α(H)β(S)[α(MD)β(MDD)] transgenic mouse lines that express high levels of human β5 globin were anesthetized and administered horseradish peroxidase (HRP) intracardially. After 1 min, the animals were sacrificed, and the retina from one eye was excised, fixed, and developed in diaminobenzidine (DAB). The contralateral eye was fixed, embedded whole in glycol methacrylate, and HRP developed in 2.5 μm sections. Results. HRP reaction product (HRP-RP) and stained erythrocytes (RBCs) (due to endogenous peroxidase) were diffusely distributed within all vascular lumens in flatmount retinas from control animals (littermates homozygous for the mouse β(Major) deletion not expressing the β5 transgene). In 42.5{\%} of the transgenic mice expressing β5 without any proliferative retinopathy, many blood vessels contained RBC plugs and lacked lumenal HRP-RP. In addition to packed RBCs, fibrin was sometimes present at sites of occlusion. In sections from whole eyes of the same animals, foci of photoreceptor degeneration were associated with areas of choriocapillaris nonperfusion (lumen that lacked HRP-PR). In areas with normal photoreceptors, the choriocapillaris appeared perfused (HRP-RP was present). In animals with proliferative chorioretinopathy, some neovascular formations lacked luminal HRP-RP, suggesting autoinfarction. Conclusions. Nonperfused retinal and choroidal vessels were observed in mice from the α(H)β(S)[β(MDD)] and (α(H)β(S)[α(MD)β(MDD)] lines without retinal and choroidal neovascularization. whereas, all mice with neovascularization had nonperfused areas. Furthermore, small foci of PR loss were associated with areas of nonperfused choriocapillaris. These results suggest that sickle cell-mediated vaso-occlusions are an initial event in the chorioretinopathy and outer retinal atrophy that occurs in these models.",
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T1 - Nonperfusion of retina and choroid in transgenic mouse models of sickle cell disease

AU - Lutty, G. A.

AU - Merges, C.

AU - McLeod, D. S.

AU - Wajer, S. D.

AU - Suzuka, S. M.

AU - Fabry, M. E.

AU - Nagel, R. L.

PY - 1998/4

Y1 - 1998/4

N2 - Purpose. To determine if vascular occlusion and nonperfusion is associated with the outer retinal atrophy, retinopathy, and choroidopathy (chorioretinopathy) that occurs in the α(H)β(S)[β(MDV)] and α(H)β(S)[α(MD)β(MDD)] transgenic mouse models of sickle cell disease. Methods. Mice from the α(H)β(S)[β(MDD)] and α(H)β(S)[α(MD)β(MDD)] transgenic mouse lines that express high levels of human β5 globin were anesthetized and administered horseradish peroxidase (HRP) intracardially. After 1 min, the animals were sacrificed, and the retina from one eye was excised, fixed, and developed in diaminobenzidine (DAB). The contralateral eye was fixed, embedded whole in glycol methacrylate, and HRP developed in 2.5 μm sections. Results. HRP reaction product (HRP-RP) and stained erythrocytes (RBCs) (due to endogenous peroxidase) were diffusely distributed within all vascular lumens in flatmount retinas from control animals (littermates homozygous for the mouse β(Major) deletion not expressing the β5 transgene). In 42.5% of the transgenic mice expressing β5 without any proliferative retinopathy, many blood vessels contained RBC plugs and lacked lumenal HRP-RP. In addition to packed RBCs, fibrin was sometimes present at sites of occlusion. In sections from whole eyes of the same animals, foci of photoreceptor degeneration were associated with areas of choriocapillaris nonperfusion (lumen that lacked HRP-PR). In areas with normal photoreceptors, the choriocapillaris appeared perfused (HRP-RP was present). In animals with proliferative chorioretinopathy, some neovascular formations lacked luminal HRP-RP, suggesting autoinfarction. Conclusions. Nonperfused retinal and choroidal vessels were observed in mice from the α(H)β(S)[β(MDD)] and (α(H)β(S)[α(MD)β(MDD)] lines without retinal and choroidal neovascularization. whereas, all mice with neovascularization had nonperfused areas. Furthermore, small foci of PR loss were associated with areas of nonperfused choriocapillaris. These results suggest that sickle cell-mediated vaso-occlusions are an initial event in the chorioretinopathy and outer retinal atrophy that occurs in these models.

AB - Purpose. To determine if vascular occlusion and nonperfusion is associated with the outer retinal atrophy, retinopathy, and choroidopathy (chorioretinopathy) that occurs in the α(H)β(S)[β(MDV)] and α(H)β(S)[α(MD)β(MDD)] transgenic mouse models of sickle cell disease. Methods. Mice from the α(H)β(S)[β(MDD)] and α(H)β(S)[α(MD)β(MDD)] transgenic mouse lines that express high levels of human β5 globin were anesthetized and administered horseradish peroxidase (HRP) intracardially. After 1 min, the animals were sacrificed, and the retina from one eye was excised, fixed, and developed in diaminobenzidine (DAB). The contralateral eye was fixed, embedded whole in glycol methacrylate, and HRP developed in 2.5 μm sections. Results. HRP reaction product (HRP-RP) and stained erythrocytes (RBCs) (due to endogenous peroxidase) were diffusely distributed within all vascular lumens in flatmount retinas from control animals (littermates homozygous for the mouse β(Major) deletion not expressing the β5 transgene). In 42.5% of the transgenic mice expressing β5 without any proliferative retinopathy, many blood vessels contained RBC plugs and lacked lumenal HRP-RP. In addition to packed RBCs, fibrin was sometimes present at sites of occlusion. In sections from whole eyes of the same animals, foci of photoreceptor degeneration were associated with areas of choriocapillaris nonperfusion (lumen that lacked HRP-PR). In areas with normal photoreceptors, the choriocapillaris appeared perfused (HRP-RP was present). In animals with proliferative chorioretinopathy, some neovascular formations lacked luminal HRP-RP, suggesting autoinfarction. Conclusions. Nonperfused retinal and choroidal vessels were observed in mice from the α(H)β(S)[β(MDD)] and (α(H)β(S)[α(MD)β(MDD)] lines without retinal and choroidal neovascularization. whereas, all mice with neovascularization had nonperfused areas. Furthermore, small foci of PR loss were associated with areas of nonperfused choriocapillaris. These results suggest that sickle cell-mediated vaso-occlusions are an initial event in the chorioretinopathy and outer retinal atrophy that occurs in these models.

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KW - Transgenic mice

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