Noninvasive diagnosis of cardiac allograft rejection

The effect of procainamide

Jeffrey E. Everett, Eric Irwin, Jose Jesserun, David P. Slovut, Sara J. Shumway

Research output: Contribution to journalArticle

Abstract

The surface electrocardiogram (ECG) has been used as a noninvasive technique for the diagnosis of cardiac allograft rejection. Alteration in conduction, R-wave amplitude, and rhythm have been associated with rejection. These ECG findings are modulated by the myocyte sodium channel, but are inconsistent and occur only during severe rejection episodes. The purpose of this study was to (I) characterize changes in cardiac electrophysiology during allograft rejection using the highly sensitive intramyocardial electrocardiogram and (2) determine whether pharmacological sodium channel blockade with procainamide enhances subtle ECG changes. Nine mongrel dogs underwent heterotopic heart transplantation in which four intramyocardial leads (one anteriorly and posteriorly on each ventricle) were attached. Leads exited to a subcutaneously placed ECG block which was transcutaneously accessed posttransplant to record direct intramyocardial electrocardiograms. Six animals were treated with procainamide. while three were not and served as controls. Daily measurements included the QRS, QT, and QTc intervals and the R-wave amplitude. Endomyocardial biopsies were performed weekly and also when significant decline in ECG amplitude occurred. Detailed ECG interval analysis failed to establish any correlation between conduction and rejection, even in the procainamide-treated group. Intramyocardial amplitude analysis, however, had a sensitivity of 100% and a specificity of 86% for the diagnosis of rejection. The results indicate that intramyocardial ECG interval analysis is not predictive of rejection even when prolonging conduction with procainamide. Amplitude analysis, however, remains an accurate noninvasive means for the early detection of cardiac allograft rejection and should allow more selective use of endomyocardial biopsy.

Original languageEnglish (US)
Pages (from-to)195-201
Number of pages7
JournalJournal of Investigative Surgery
Volume8
Issue number3
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Procainamide
Allografts
Electrocardiography
Sodium Channels
Cardiac Electrophysiology
Heterotopic Transplantation
Biopsy
Heart Transplantation
Muscle Cells
Pharmacology
Dogs

Keywords

  • Cardiac electrophysiology
  • Noninvasive detection rejection
  • Transplantation

ASJC Scopus subject areas

  • Surgery

Cite this

Noninvasive diagnosis of cardiac allograft rejection : The effect of procainamide. / Everett, Jeffrey E.; Irwin, Eric; Jesserun, Jose; Slovut, David P.; Shumway, Sara J.

In: Journal of Investigative Surgery, Vol. 8, No. 3, 1995, p. 195-201.

Research output: Contribution to journalArticle

Everett, Jeffrey E. ; Irwin, Eric ; Jesserun, Jose ; Slovut, David P. ; Shumway, Sara J. / Noninvasive diagnosis of cardiac allograft rejection : The effect of procainamide. In: Journal of Investigative Surgery. 1995 ; Vol. 8, No. 3. pp. 195-201.
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