Nondigest liberation of biomarkers from plasma

A novel two-stage ultrafiltration approach

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A simple procedure for sample preparation of human plasma by two stages of ultra-filtration using one device is described. Our approach is useful for nondigest liberation of biomarkers bound to albumin and other plasma proteins. The analyte contained in the ultrafiltrate can be directly analyzed without additional sample preparation, and quantified by 2-D RP-RP LC/MS.

Original languageEnglish (US)
Pages (from-to)245-248
Number of pages4
JournalJournal of Separation Science
Volume30
Issue number2
DOIs
StatePublished - Feb 2007

Fingerprint

Plasma (human)
Ultrafiltration
Biomarkers
Blood Proteins
Albumins
Proteins
Plasmas
Equipment and Supplies

Keywords

  • 2-D chromatography
  • Albumin
  • Biomarkers
  • LC/MS
  • Ultrafiltration

ASJC Scopus subject areas

  • Molecular Medicine
  • Clinical Biochemistry
  • Chemical Engineering(all)
  • Filtration and Separation

Cite this

Nondigest liberation of biomarkers from plasma : A novel two-stage ultrafiltration approach. / Rogatsky, Eduard; Tomuta, Vlad A.; Stein, Daniel T.

In: Journal of Separation Science, Vol. 30, No. 2, 02.2007, p. 245-248.

Research output: Contribution to journalArticle

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