TY - JOUR
T1 - Non-core subunit eIF3h of translation initiation factor eIF3 regulates zebrafish embryonic development
AU - Choudhuri, Avik
AU - Evans, Todd
AU - Maitra, Umadas
N1 - Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2010/6
Y1 - 2010/6
N2 - Eukaryotic translation initiation factor eIF3, which plays a central role in translation initiation, consists of five core subunits that are present in both the budding yeast and higher eukaryotes. However, higher eukaryotic eIF3 contains additional (non-core) subunits that are absent in the budding yeast. We investigated the role of one such non-core eIF3 subunit eIF3h, encoded by two distinct genes - eif3ha and eif3hb, as a regulator of embryonic development in zebrafish. Both eif3h genes are expressed during early embryogenesis, and display overlapping yet distinct and highly dynamic spatial expression patterns. Loss of function analysis using specific morpholino oligomers indicates that each isoform has specific as well as redundant functions during early development. The morphant phenotypes correlate with their spatial expression patterns, indicating that eif3h regulates development of the brain, heart, vasculature, and lateral line. These results indicate that the non-core subunits of eIF3 regulate specific developmental programs during vertebrate embryogenesis.
AB - Eukaryotic translation initiation factor eIF3, which plays a central role in translation initiation, consists of five core subunits that are present in both the budding yeast and higher eukaryotes. However, higher eukaryotic eIF3 contains additional (non-core) subunits that are absent in the budding yeast. We investigated the role of one such non-core eIF3 subunit eIF3h, encoded by two distinct genes - eif3ha and eif3hb, as a regulator of embryonic development in zebrafish. Both eif3h genes are expressed during early embryogenesis, and display overlapping yet distinct and highly dynamic spatial expression patterns. Loss of function analysis using specific morpholino oligomers indicates that each isoform has specific as well as redundant functions during early development. The morphant phenotypes correlate with their spatial expression patterns, indicating that eif3h regulates development of the brain, heart, vasculature, and lateral line. These results indicate that the non-core subunits of eIF3 regulate specific developmental programs during vertebrate embryogenesis.
KW - Eukaryotic translation initiation
KW - Regulation of early embryogenesis by eIF3h
KW - Translation initiation factor 3h subunit
KW - Zebrafish development
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U2 - 10.1002/dvdy.22289
DO - 10.1002/dvdy.22289
M3 - Article
C2 - 20503360
AN - SCOPUS:77952706884
VL - 239
SP - 1632
EP - 1644
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 6
ER -