NO reactions with sol-gel and solution phase samples of the ferric nitrite derivative of HbA

Camille J. Roche, Joel M. Friedman

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The reaction of nitric oxide (NO) with the ferric (met) nitrite derivative of human adult hemoglobin Hb is probed for both solution phase and sol-gel encapsulated populations. The evolution of both the Q band absorption spectrum and fitted populations of Hb derivatives are used to show the sequence of events occurring when NO interacts with nitrite bound to a ferric heme in Hb. The sol-gel is used to compare the evolving populations as a function of quaternary state for the starting met-nitrite populations. The redox status of intermediates is probed using the CN- anion to trap ferric heme species. The emergent presence of reactive NO species such as N2O3 during the course of the reaction is probed using the fluorescent probe DAF-2 whereas the fluorophore Chemifluor is used as an indirect measure of the ability of the reaction to create S-nitrosothiols on glutathione. The results are consistent with the formation of a stable reactive intermediate capable of generating bioactive forms of NO. The patterns observed are consistent with a proposed mechanism whereby NO reacts with the ferric nitrite derivative to generate N2O3.

Original languageEnglish (US)
Pages (from-to)180-190
Number of pages11
JournalNitric Oxide - Biology and Chemistry
Volume22
Issue number2
DOIs
StatePublished - Feb 15 2010

Keywords

  • Dinitrogentrioxide
  • Hemoglobin
  • Nitric oxide
  • Nitrite
  • S-nitrosolation
  • Sol-gel

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Clinical Biochemistry
  • Cancer Research

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