NO Candida auris: Nitric oxide in nanotherapeutics to combat emerging fungal pathogen Candida auris

Levi G. Cleare, Kevin L. Li, Waleed M. Abuzeid, Parimala Nacharaju, Joel M. Friedman, Joshua D. Nosanchuk

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Candida auris (C. auris) is an emerging pathogenic fungal species that is especially worrisome due to its high mortality rates and widespread antifungal resistance. Previous studies have demonstrated the efficacy of nitric oxide (NO) nanoparticles on Candida species, and, to our knowledge, this is the first study to investigate the antifungal effects of a NO-generating nanoparticle on C. auris. Six C. auris strains were incubated with a nanoparticle (NAC-SNO-np), which releases N-acetylcysteine S-nitrosothiol (NAC-SNO) and N-acetylcysteine (NAC), and generates NO, through colony forming unit (CFU) assays, and confocal laser scanning microscopy. NAC-SNO-np effectively eradicates planktonic and biofilm C. auris. Across all six strains, 10 mg/mL NAC-SNO-np significantly reduced the number of CFUs (p < 0.05) and demonstrated a >70% decrease in biofilm viability (p < 0.05). NAC-SNO-np effectively eradicates planktonic C. auris and significantly reduces C. auris biofilm formation. Hence, this novel NO-releasing nanoparticle shows promise as a future therapeutic.

Original languageEnglish (US)
Article number85
Pages (from-to)1-13
Number of pages13
JournalJournal of Fungi
Volume6
Issue number2
DOIs
StatePublished - Jun 2020

Keywords

  • C. auris
  • Candida auris
  • Confocal microscopy
  • NAC
  • Nanoparticles
  • Nitric oxide

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Microbiology (medical)

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