Abstract
NLRP10 is a nucleotide-binding oligomerization domain-like receptor that functions as an intracellular pattern recognition receptor for microbial products. Here, we generated a Nlrp10-/- mouse to delineate the role of NLRP10 in the host immune response and found that Nlrp10-/- dendritic cells (DCs) elicited sub-optimal IFNγ production by antigen-specific CD4+ T cells compared to wild-type (WT) DCs. In response to T-cell encounter, CD40 ligation or Toll-like receptor 9 stimulation, Nlrp10-/- DCs produced low levels of IL-12, due to a substantial decrease in NF-κB activation. Defective IL-12 production was also evident in vivo and affected IFNγ production by CD4+ T cells. Upon Mycobacterium tuberculosis (Mtb) infection, Nlrp10-/- mice displayed diminished T helper 1-cell responses and increased bacterial growth compared to WT mice. These data indicate that NLRP10-mediated IL-12 production by DCs is critical for IFNγ induction in T cells and contributes to promote the host defense against Mtb.
Original language | English (US) |
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Article number | 1462 |
Journal | Frontiers in immunology |
Volume | 8 |
Issue number | NOV |
DOIs | |
State | Published - Nov 2 2017 |
Keywords
- CpG DNA
- Dendritic cells
- IFNγ
- IL-12
- Mycobacterium tuberculosis
- NLRP10
- T helper 1
- Toll-like receptor 9
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology