Nifedipine protects small intestine from cyclosporine-induced hemodynamic and functional impairment

Shuching Sun, Stuart M. Greenstein, Dean Y. Kim, Thomas C. Schreiber, Richard S. Schechner, Vivian A. Tellis

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have previously shown that cyclosporine (CsA) causes intestinal hemodynamic and functional impairments. In this study, we evaluated whether nifedipine protects the small intestine from such toxic side effects. Isogeneic small intestinal transplantation was performed in rats which then received one of the following two-week treatments: olive oil, 0.15 ml/kg/day i.m. as vehicle controls in group 1; nifedipine, 1 mg/kg/day i.m. in group 2; CsA, 15 mg/kg/day i.m. in group 3; and both nifedipine and CsA in group 4. Vascular resistance, whole tissue blood flow and its mucosal and serosal/muscularis distributions in both graft and recipient residual native intestines, and absorptive function were determined. The data showed that two-week treatment with CsA resulted in a marked elevation of vascular resistance from 51.0 ± 6.8 to 72.4 ± 11.1 U/g in the native whole tissue and from 53.7 ± 7.2 to 78.2 ± 12.1 U/g in the graft whole tissue, and decreases in blood flow from 1.59 ± 0.26 to 1.11 ± 0.17 ml/g/min in the native whole tissue and from 1.50 ± 0.21 to 1.03 ± 0.18 ml/g/min in the graft whole tissue and absorption from 227 ± 36 to 166 ± 26 mg glucose/dl. Mucosa was preferentially affected, while serosal/muscularis layers remained relatively unchanged. When nifedipine was concomitantly used with CsA, vascular resistance and blood flow values in the mucosal layer and whole intestinal tissue as well as absorptive function showed no significant differences from the baseline data. The changes observed in denervated grafts and recipient native intestines were similar. We conclude that nifedipine is effective in protecting both graft and native small intestines from CsA- induced toxicity in the rat.

Original languageEnglish (US)
Pages (from-to)295-299
Number of pages5
JournalJournal of Surgical Research
Volume69
Issue number2
DOIs
StatePublished - May 1997

Fingerprint

Nifedipine
Cyclosporine
Small Intestine
Hemodynamics
Transplants
Vascular Resistance
Intestines
Poisons
Mucous Membrane
Transplantation
Glucose
Control Groups

ASJC Scopus subject areas

  • Surgery

Cite this

Nifedipine protects small intestine from cyclosporine-induced hemodynamic and functional impairment. / Sun, Shuching; Greenstein, Stuart M.; Kim, Dean Y.; Schreiber, Thomas C.; Schechner, Richard S.; Tellis, Vivian A.

In: Journal of Surgical Research, Vol. 69, No. 2, 05.1997, p. 295-299.

Research output: Contribution to journalArticle

Sun, Shuching ; Greenstein, Stuart M. ; Kim, Dean Y. ; Schreiber, Thomas C. ; Schechner, Richard S. ; Tellis, Vivian A. / Nifedipine protects small intestine from cyclosporine-induced hemodynamic and functional impairment. In: Journal of Surgical Research. 1997 ; Vol. 69, No. 2. pp. 295-299.
@article{a689736c3f5c45d5bd8ef00095a54c43,
title = "Nifedipine protects small intestine from cyclosporine-induced hemodynamic and functional impairment",
abstract = "We have previously shown that cyclosporine (CsA) causes intestinal hemodynamic and functional impairments. In this study, we evaluated whether nifedipine protects the small intestine from such toxic side effects. Isogeneic small intestinal transplantation was performed in rats which then received one of the following two-week treatments: olive oil, 0.15 ml/kg/day i.m. as vehicle controls in group 1; nifedipine, 1 mg/kg/day i.m. in group 2; CsA, 15 mg/kg/day i.m. in group 3; and both nifedipine and CsA in group 4. Vascular resistance, whole tissue blood flow and its mucosal and serosal/muscularis distributions in both graft and recipient residual native intestines, and absorptive function were determined. The data showed that two-week treatment with CsA resulted in a marked elevation of vascular resistance from 51.0 ± 6.8 to 72.4 ± 11.1 U/g in the native whole tissue and from 53.7 ± 7.2 to 78.2 ± 12.1 U/g in the graft whole tissue, and decreases in blood flow from 1.59 ± 0.26 to 1.11 ± 0.17 ml/g/min in the native whole tissue and from 1.50 ± 0.21 to 1.03 ± 0.18 ml/g/min in the graft whole tissue and absorption from 227 ± 36 to 166 ± 26 mg glucose/dl. Mucosa was preferentially affected, while serosal/muscularis layers remained relatively unchanged. When nifedipine was concomitantly used with CsA, vascular resistance and blood flow values in the mucosal layer and whole intestinal tissue as well as absorptive function showed no significant differences from the baseline data. The changes observed in denervated grafts and recipient native intestines were similar. We conclude that nifedipine is effective in protecting both graft and native small intestines from CsA- induced toxicity in the rat.",
author = "Shuching Sun and Greenstein, {Stuart M.} and Kim, {Dean Y.} and Schreiber, {Thomas C.} and Schechner, {Richard S.} and Tellis, {Vivian A.}",
year = "1997",
month = "5",
doi = "10.1006/jsre.1997.5035",
language = "English (US)",
volume = "69",
pages = "295--299",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Nifedipine protects small intestine from cyclosporine-induced hemodynamic and functional impairment

AU - Sun, Shuching

AU - Greenstein, Stuart M.

AU - Kim, Dean Y.

AU - Schreiber, Thomas C.

AU - Schechner, Richard S.

AU - Tellis, Vivian A.

PY - 1997/5

Y1 - 1997/5

N2 - We have previously shown that cyclosporine (CsA) causes intestinal hemodynamic and functional impairments. In this study, we evaluated whether nifedipine protects the small intestine from such toxic side effects. Isogeneic small intestinal transplantation was performed in rats which then received one of the following two-week treatments: olive oil, 0.15 ml/kg/day i.m. as vehicle controls in group 1; nifedipine, 1 mg/kg/day i.m. in group 2; CsA, 15 mg/kg/day i.m. in group 3; and both nifedipine and CsA in group 4. Vascular resistance, whole tissue blood flow and its mucosal and serosal/muscularis distributions in both graft and recipient residual native intestines, and absorptive function were determined. The data showed that two-week treatment with CsA resulted in a marked elevation of vascular resistance from 51.0 ± 6.8 to 72.4 ± 11.1 U/g in the native whole tissue and from 53.7 ± 7.2 to 78.2 ± 12.1 U/g in the graft whole tissue, and decreases in blood flow from 1.59 ± 0.26 to 1.11 ± 0.17 ml/g/min in the native whole tissue and from 1.50 ± 0.21 to 1.03 ± 0.18 ml/g/min in the graft whole tissue and absorption from 227 ± 36 to 166 ± 26 mg glucose/dl. Mucosa was preferentially affected, while serosal/muscularis layers remained relatively unchanged. When nifedipine was concomitantly used with CsA, vascular resistance and blood flow values in the mucosal layer and whole intestinal tissue as well as absorptive function showed no significant differences from the baseline data. The changes observed in denervated grafts and recipient native intestines were similar. We conclude that nifedipine is effective in protecting both graft and native small intestines from CsA- induced toxicity in the rat.

AB - We have previously shown that cyclosporine (CsA) causes intestinal hemodynamic and functional impairments. In this study, we evaluated whether nifedipine protects the small intestine from such toxic side effects. Isogeneic small intestinal transplantation was performed in rats which then received one of the following two-week treatments: olive oil, 0.15 ml/kg/day i.m. as vehicle controls in group 1; nifedipine, 1 mg/kg/day i.m. in group 2; CsA, 15 mg/kg/day i.m. in group 3; and both nifedipine and CsA in group 4. Vascular resistance, whole tissue blood flow and its mucosal and serosal/muscularis distributions in both graft and recipient residual native intestines, and absorptive function were determined. The data showed that two-week treatment with CsA resulted in a marked elevation of vascular resistance from 51.0 ± 6.8 to 72.4 ± 11.1 U/g in the native whole tissue and from 53.7 ± 7.2 to 78.2 ± 12.1 U/g in the graft whole tissue, and decreases in blood flow from 1.59 ± 0.26 to 1.11 ± 0.17 ml/g/min in the native whole tissue and from 1.50 ± 0.21 to 1.03 ± 0.18 ml/g/min in the graft whole tissue and absorption from 227 ± 36 to 166 ± 26 mg glucose/dl. Mucosa was preferentially affected, while serosal/muscularis layers remained relatively unchanged. When nifedipine was concomitantly used with CsA, vascular resistance and blood flow values in the mucosal layer and whole intestinal tissue as well as absorptive function showed no significant differences from the baseline data. The changes observed in denervated grafts and recipient native intestines were similar. We conclude that nifedipine is effective in protecting both graft and native small intestines from CsA- induced toxicity in the rat.

UR - http://www.scopus.com/inward/record.url?scp=0031147771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031147771&partnerID=8YFLogxK

U2 - 10.1006/jsre.1997.5035

DO - 10.1006/jsre.1997.5035

M3 - Article

VL - 69

SP - 295

EP - 299

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 2

ER -