Next-generation rapid autopsies enable tumor evolution tracking andgeneration of preclinical models

David J. Pisapia, Steven Salvatore, Chantal Pauli, Erika Hissong, Ken Eng, Davide Prandi, Verena Wilbeth Sailer, Brian D. Robinson, Kyung Park, Joanna Cyrta, Scott T. Tagawa, Myriam Kossai, Jacqueline Fontugne, Robert Kim, Alexandros Sigaras, Rema Rao, Danielle Pancirer, Bishoy Faltas, Rohan Bareja, Ana M. MolinaDavid M. Nanus, Prajwal Rajappa, Mark M. Souweidane, Jeffrey Greenfield, Anne Katrin Emde, Nicolas Robine, Olivier Elemento, Andrea Sboner, Francesca Demichelis, Himisha Beltran, Mark A. Rubin, Juan Miguel Mosquera

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Purpose Patients with cancer who graciously consent for autopsy represent an invaluable resource for the study of cancer biology. To advance the study of tumor evolution, metastases, and resistance to treatment, we developed a next-generation rapid autopsy program integrated within a broader precision medicine clinical trial that interrogates pre- and postmortem tissue samples for patients of all ages and cancer types. Materials and Methods One hundred twenty-three (22%) of 554 patients who consented to the clinical trial also consented for rapid autopsy. This report comprises the first 15 autopsies, including patients with metastatic carcinoma (n = 10), melanoma (n = 1), and glioma (n = 4). Whole-exome sequencing (WES) was performed on frozen autopsy tumor samples from multiple anatomic sites and on non-neoplastic tissue. RNA sequencing (RNA-Seq) was performed on a subset of frozen samples. Tissue was also used for the development of preclinical models, including tumor organoids and patient-derived xenografts. Results Three hundred forty-six frozen samples were procured in total.WESwas performed on 113 samples and RNA-Seq on 72 samples. Successful cell strain, tumor organoid, and/or patientderived xenograft development was achieved in four samples, including an inoperable pediatric glioma.WESdata were used to assess clonal evolution and molecular heterogeneity of tumors in individual patients. Mutational profiles of primary tumors and metastases yielded candidate mediators of metastatic spread and organotropism including CUL9 and PIGM in metastatic ependymomaandANKRD52in metastaticmelanomato the lung.RNA-Seqdata identified novel gene fusion candidates. Conclusion A next-generation sequencing-based autopsy program in conjunction with a premortem precision medicine pipeline for diverse tumors affords a valuable window into clonal evolution, metastasis, and alterations underlying treatment. Moreover, such an autopsy program yields robust preclinical models of disease.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalJCO Precision Oncology
Issue number1
StatePublished - 2017
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


Dive into the research topics of 'Next-generation rapid autopsies enable tumor evolution tracking andgeneration of preclinical models'. Together they form a unique fingerprint.

Cite this