New protocol in spotting microarray technique.

A. D. Iacobaş, M. Urban, David C. Spray

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The "spotting" microarray technique, consisting in large sets of DNA sequences spotted on poly-L-lysine-coated glass microscope slides, has been developed to comparatively analyze genome-wide patterns of mRNA expression. It is now a valuable tool employed in order to quantitatively monitor gene expression profiles, as well as to analyze the alterations produced in case of genetic diseases, or induced by different treatments, abnormal nutrition and toxin. Our group improved the standard protocol as well as the results spreadsheet, adding new experiments and mathematical processing procedures in order to increase the accuracy of the data and get new information. In this contribution, we propose and verify two procedures to correct the spot ratios and a new protocol to get the normal variability of the digital gene expression.

Original languageEnglish (US)
Pages (from-to)69-80
Number of pages12
JournalRomanian journal of physiology : physiological sciences / [Academia de Stiinte Medicale]
Volume37
Issue number1-4
StatePublished - 2000

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Metrorrhagia
Inborn Genetic Diseases
Transcriptome
Lysine
Glass
Genome
Gene Expression
Messenger RNA
Data Accuracy

Cite this

New protocol in spotting microarray technique. / Iacobaş, A. D.; Urban, M.; Spray, David C.

In: Romanian journal of physiology : physiological sciences / [Academia de Stiinte Medicale], Vol. 37, No. 1-4, 2000, p. 69-80.

Research output: Contribution to journalArticle

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