New methods for inducing the differentiation of amniotic-derived mesenchymal stem cells into motor neuron precursor cells

Wei Hu, Fangxia Guan, Yuan Li, You jia Tang, Feng Yang, Bo Yang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Our study investigates the differentiation of amniotic-derived mesenchymal stem cells (ADMSCs) into motor neuron (MN) precursor cells induced by a combination of extracellular matrix (ECM) and multi-cell factors. Membrane-like ECM was made by an enzymatic and chemical extraction method and exhibited good biological compatibility. Cells in the experimental group (EG) were treated with ECM and multi-cell factors in a multi-step induction process, while the control group (CG) was treated similarly, except without ECM. In the EG, after induction, the cells formed processes that connected with neighboring cells to form a net that had directionality. In these cells, neuron-specific enolase (NSE) and synaptophysin (SYN) expression levels increased and glial fibrillary acidic protein (GFAP) expression decreased. The SYN expression in the EG cells was higher compared with those in the CG. In the CG, NSE expression increased, while the expression of Nestin and SYN did not change. These were several changes in the levels of other genes: ADMSCs at passage 1 expressed Nanog, SOX2, octamer-binding transcription factor 4 (OCT4) and Nestin. In the EG, at the beginning of induction, the expression of Nanog decreased and that of SOX2 and Nestin increased. After 2 days, the cells expressed Nestin, OCT4 and SYNIII, and after 3 days, they expressed Olig2, OCT4, Nestin, SYNII and Islet1 (ISL1). Finally, at day 6, the cells expressed Nestin, SYNI, SYNIII, ISL-1, homeobox 9 (Hb9) and oligodendrocyte lineage transcription factor 2 (Olig2). In the CG, the cells never expressed SYNI, SYNII or Hb9. Our studies therefore demonstrate that the extracted ECM was capable of promoting the maturation of synapses. Human ADMSCs are composed of multiple cell subsets, including neural progenitor cells. The multi-step induction method used in this study causes human ADMSCs to differentiate into MN precursor cells.

Original languageEnglish (US)
Pages (from-to)295-305
Number of pages11
JournalTissue and Cell
Volume45
Issue number5
DOIs
StatePublished - Oct 2013
Externally publishedYes

Fingerprint

Motor Neurons
Mesenchymal Stromal Cells
Nestin
Octamer Transcription Factors
Extracellular Matrix
Synaptophysin
Control Groups
Phosphopyruvate Hydratase
Homeobox Genes
Glial Fibrillary Acidic Protein
Oligodendroglia
Synapses
Transcription Factors
Stem Cells

Keywords

  • Amniotic-derived mesenchymal stem cells
  • Differentiation
  • Extracellular matrix
  • MN precursor cells

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

Cite this

New methods for inducing the differentiation of amniotic-derived mesenchymal stem cells into motor neuron precursor cells. / Hu, Wei; Guan, Fangxia; Li, Yuan; Tang, You jia; Yang, Feng; Yang, Bo.

In: Tissue and Cell, Vol. 45, No. 5, 10.2013, p. 295-305.

Research output: Contribution to journalArticle

Hu, Wei ; Guan, Fangxia ; Li, Yuan ; Tang, You jia ; Yang, Feng ; Yang, Bo. / New methods for inducing the differentiation of amniotic-derived mesenchymal stem cells into motor neuron precursor cells. In: Tissue and Cell. 2013 ; Vol. 45, No. 5. pp. 295-305.
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