New melanocortin-like peptide of E. coli can suppress inflammation via the mammalian melanocortin-1 receptor (MC1R): Possible endocrine-like function for microbes of the gut

Xiaoling Qiang, Anthony S. Liotta, Joseph Shiloach, J. C. Gutierrez, Haichao Wang, Mahendar Ochani, Kanta Ochani, Huan Yang, Aviva Rabin, Derek Leroith, Maxine A. Lesniak, Markus Böhm, Christian Maaser, Klaus Kannengiesser, Mark Donowitz, Shervin Rabizadeh, Christopher J. Czura, Kevin J. Tracey, Mark Westlake, Aida ZarfeshaniSyed F. Mehdi, Ann Danoff, Xueliang Ge, Suparna Sanyal, Gary J. Schwartz, Jesse Roth

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

E. coli releases a 33 amino acid peptide melanocortin-like peptide of E. coli (MECO-1) that is identical to the C-terminus of the E. coli elongation factor-G (EF-G) and has interesting similarities to two prominent mammalian melanocortin hormones, alpha-melanocyte-stimulating hormone (alpha-MSH) and adrenocorticotropin (ACTH). Note that MECO-1 lacks HFRW, the common pharmacophore of the known mammalian melanocortin peptides. MECO-1 and the two hormones were equally effective in severely blunting release of cytokines (HMGB1 and TNF) from macrophage-like cells in response to (i) endotoxin (lipopolysaccharide) or (ii) pro-inflammatory cytokine HMGB-1. The in vitro anti-inflammatoty effects of MECO-1 and of alpha-MSH were abrogated by (i) antibody against melanocortin-1 receptor (MC1R) and by (ii) agouti, an endogenous inverse agonist of MC1R. In vivo MECO-1 was even more potent than alpha-MSH in rescuing mice from death due to (i) lethal doses of LPS endotoxin or (ii) cecal ligation and puncture, models of sterile and infectious sepsis, respectively.

Original languageEnglish (US)
Article number31
Journalnpj Biofilms and Microbiomes
Volume3
Issue number1
DOIs
StatePublished - Dec 1 2017

Fingerprint

Receptor, Melanocortin, Type 1
Melanocortins
alpha-MSH
Escherichia coli
Inflammation
Endotoxins
Adrenocorticotropic Hormone
HMGB Proteins
Peptide Elongation Factor G
Hormones
Cytokines
HMGB1 Protein
Peptides
Punctures
Ligation
Lipopolysaccharides
Sepsis
Macrophages
Amino Acids
Antibodies

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

New melanocortin-like peptide of E. coli can suppress inflammation via the mammalian melanocortin-1 receptor (MC1R) : Possible endocrine-like function for microbes of the gut. / Qiang, Xiaoling; Liotta, Anthony S.; Shiloach, Joseph; Gutierrez, J. C.; Wang, Haichao; Ochani, Mahendar; Ochani, Kanta; Yang, Huan; Rabin, Aviva; Leroith, Derek; Lesniak, Maxine A.; Böhm, Markus; Maaser, Christian; Kannengiesser, Klaus; Donowitz, Mark; Rabizadeh, Shervin; Czura, Christopher J.; Tracey, Kevin J.; Westlake, Mark; Zarfeshani, Aida; Mehdi, Syed F.; Danoff, Ann; Ge, Xueliang; Sanyal, Suparna; Schwartz, Gary J.; Roth, Jesse.

In: npj Biofilms and Microbiomes, Vol. 3, No. 1, 31, 01.12.2017.

Research output: Contribution to journalArticle

Qiang, X, Liotta, AS, Shiloach, J, Gutierrez, JC, Wang, H, Ochani, M, Ochani, K, Yang, H, Rabin, A, Leroith, D, Lesniak, MA, Böhm, M, Maaser, C, Kannengiesser, K, Donowitz, M, Rabizadeh, S, Czura, CJ, Tracey, KJ, Westlake, M, Zarfeshani, A, Mehdi, SF, Danoff, A, Ge, X, Sanyal, S, Schwartz, GJ & Roth, J 2017, 'New melanocortin-like peptide of E. coli can suppress inflammation via the mammalian melanocortin-1 receptor (MC1R): Possible endocrine-like function for microbes of the gut', npj Biofilms and Microbiomes, vol. 3, no. 1, 31. https://doi.org/10.1038/s41522-017-0039-9
Qiang, Xiaoling ; Liotta, Anthony S. ; Shiloach, Joseph ; Gutierrez, J. C. ; Wang, Haichao ; Ochani, Mahendar ; Ochani, Kanta ; Yang, Huan ; Rabin, Aviva ; Leroith, Derek ; Lesniak, Maxine A. ; Böhm, Markus ; Maaser, Christian ; Kannengiesser, Klaus ; Donowitz, Mark ; Rabizadeh, Shervin ; Czura, Christopher J. ; Tracey, Kevin J. ; Westlake, Mark ; Zarfeshani, Aida ; Mehdi, Syed F. ; Danoff, Ann ; Ge, Xueliang ; Sanyal, Suparna ; Schwartz, Gary J. ; Roth, Jesse. / New melanocortin-like peptide of E. coli can suppress inflammation via the mammalian melanocortin-1 receptor (MC1R) : Possible endocrine-like function for microbes of the gut. In: npj Biofilms and Microbiomes. 2017 ; Vol. 3, No. 1.
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abstract = "E. coli releases a 33 amino acid peptide melanocortin-like peptide of E. coli (MECO-1) that is identical to the C-terminus of the E. coli elongation factor-G (EF-G) and has interesting similarities to two prominent mammalian melanocortin hormones, alpha-melanocyte-stimulating hormone (alpha-MSH) and adrenocorticotropin (ACTH). Note that MECO-1 lacks HFRW, the common pharmacophore of the known mammalian melanocortin peptides. MECO-1 and the two hormones were equally effective in severely blunting release of cytokines (HMGB1 and TNF) from macrophage-like cells in response to (i) endotoxin (lipopolysaccharide) or (ii) pro-inflammatory cytokine HMGB-1. The in vitro anti-inflammatoty effects of MECO-1 and of alpha-MSH were abrogated by (i) antibody against melanocortin-1 receptor (MC1R) and by (ii) agouti, an endogenous inverse agonist of MC1R. In vivo MECO-1 was even more potent than alpha-MSH in rescuing mice from death due to (i) lethal doses of LPS endotoxin or (ii) cecal ligation and puncture, models of sterile and infectious sepsis, respectively.",
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T2 - Possible endocrine-like function for microbes of the gut

AU - Qiang, Xiaoling

AU - Liotta, Anthony S.

AU - Shiloach, Joseph

AU - Gutierrez, J. C.

AU - Wang, Haichao

AU - Ochani, Mahendar

AU - Ochani, Kanta

AU - Yang, Huan

AU - Rabin, Aviva

AU - Leroith, Derek

AU - Lesniak, Maxine A.

AU - Böhm, Markus

AU - Maaser, Christian

AU - Kannengiesser, Klaus

AU - Donowitz, Mark

AU - Rabizadeh, Shervin

AU - Czura, Christopher J.

AU - Tracey, Kevin J.

AU - Westlake, Mark

AU - Zarfeshani, Aida

AU - Mehdi, Syed F.

AU - Danoff, Ann

AU - Ge, Xueliang

AU - Sanyal, Suparna

AU - Schwartz, Gary J.

AU - Roth, Jesse

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