New insights and updated guidelines for epigenome-wide association studies

Lisa H. Chadwick, Akira Sawa, Ivana V. Yang, Andrea Baccarelli, Xandra O. Breakefield, Hong Wen Deng, Dana C. Dolinoy, M. Daniele Fallin, Nina T. Holland, E. Andres Houseman, Stavros Lomvardas, Mahendra Rao, John S. Satterlee, Frederick L. Tyson, Pandurangan Vijayanand, John M. Greally

Research output: Contribution to journalReview article

17 Scopus citations

Abstract

Epigenetic dysregulation in disease is increasingly studied as a potential mediator of pathophysiology. The epigenetic events are believed to occur in somatic cells, but the limited changes of DNA methylation in studies to date indicate that only subsets of the cells tested undergo epigenetic dysregulation. The recognition of this subpopulation effect indicates the need for care in design and execution of epigenome-wide association studies (EWASs), paying particular attention to confounding sources of variability. To maximize the sensitivity of the EWASs, ideally, the cell type mediating the disease should be tested, which is not always practical or ethical in human subjects. The value of using accessible cells as surrogates for the target, disease-mediating cell type has not been rigorously tested to date. In this review, participants in a workshop convened by the National Institutes of Health update EWAS design and execution guidelines to reflect new insights in the field.

Original languageEnglish (US)
Pages (from-to)14-19
Number of pages6
JournalNeuroepigenetics
Volume1
DOIs
StatePublished - Jan 1 2015

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Keywords

  • Association
  • DNA methylation
  • Disease
  • Epigenetic
  • Genome-wide

ASJC Scopus subject areas

  • Biochemistry
  • Developmental Neuroscience
  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience
  • Biological Psychiatry

Cite this

Chadwick, L. H., Sawa, A., Yang, I. V., Baccarelli, A., Breakefield, X. O., Deng, H. W., Dolinoy, D. C., Fallin, M. D., Holland, N. T., Houseman, E. A., Lomvardas, S., Rao, M., Satterlee, J. S., Tyson, F. L., Vijayanand, P., & Greally, J. M. (2015). New insights and updated guidelines for epigenome-wide association studies. Neuroepigenetics, 1, 14-19. https://doi.org/10.1016/j.nepig.2014.10.004