New approaches to in-cell detection of protein activity

Genetically encoded chemiluminescence probes pave the way to robust HTS assays

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

New genetically encoded biosensors utilizing the modified firefly luciferase promise a great improvement in the signal-to-noise ratio and the dynamic range of response in living cells. These biosensors are particularly suitable for high-throughput screening assays that use large-well-capacity formats because of their excellent response characteristics. The biosensor design strategies are highly generalizable and will be extremely valuable for expanding the repertoire of screenable targets in living cell systems.

Original languageEnglish (US)
Pages (from-to)335-337
Number of pages3
JournalACS Chemical Biology
Volume3
Issue number6
DOIs
StatePublished - Jun 20 2008

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Chemiluminescence
Biosensing Techniques
Luminescence
Biosensors
Assays
Cells
High-Throughput Screening Assays
Firefly Luciferases
Proteins
Signal-To-Noise Ratio
Signal to noise ratio
Screening
Throughput

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

Cite this

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abstract = "New genetically encoded biosensors utilizing the modified firefly luciferase promise a great improvement in the signal-to-noise ratio and the dynamic range of response in living cells. These biosensors are particularly suitable for high-throughput screening assays that use large-well-capacity formats because of their excellent response characteristics. The biosensor design strategies are highly generalizable and will be extremely valuable for expanding the repertoire of screenable targets in living cell systems.",
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