Neutrophil-platelet interactions in sepsis

Mohammed Aziz, Linda A. Kirschenbaum, Mark E. Astiz, Dhanonjoy C. Saha, Eric C. Rackow

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Introduction: Platelet-neutrophil interactions may contribute to impaired microvascular blood flow in septic shock. Methods: We studied 10 patients with severe sepsis and septic shock (SS), 8 critically ill non infected patients (CINS), and 7 healthy control (C) subjects. White blood cells (WBC) and red blood cells (RBC) were isolated from whole blood and were reconstituted in different combinations using WBC at a concentration of 9×103mm3, RBC at hematocrit of 25% and platelet poor plasma (PPP) or platelet rich plasma (PRP). Cell suspensions were infused at 1 ml/sec through 5u pore filters. The maximal inflection point of the pressure-time curve, Pi (mm Hg) , is an index of the relative resistance of the cell suspension to filtration. Platelet activation and platelet- neutrophil interactions were assessed using monoclonal antibodies (MoAb) to CD41 and activated CD62 platelet receptors. The intensity of MoAb immunoflourescence of the leukocyte subgroup was used as an index of platelet-neutrophil adhesion. Data are expressed as mean ± standard error, * p

Original languageEnglish (US)
JournalCritical Care Medicine
Volume27
Issue number1 SUPPL.
StatePublished - 1999
Externally publishedYes

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Sepsis
Neutrophils
Blood Platelets
Leukocytes
Septic Shock
Suspensions
Erythrocytes
Monoclonal Antibodies
Platelet-Rich Plasma
Platelet Activation
Hematocrit
Critical Illness
Pressure

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Aziz, M., Kirschenbaum, L. A., Astiz, M. E., Saha, D. C., & Rackow, E. C. (1999). Neutrophil-platelet interactions in sepsis. Critical Care Medicine, 27(1 SUPPL.).

Neutrophil-platelet interactions in sepsis. / Aziz, Mohammed; Kirschenbaum, Linda A.; Astiz, Mark E.; Saha, Dhanonjoy C.; Rackow, Eric C.

In: Critical Care Medicine, Vol. 27, No. 1 SUPPL., 1999.

Research output: Contribution to journalArticle

Aziz, M, Kirschenbaum, LA, Astiz, ME, Saha, DC & Rackow, EC 1999, 'Neutrophil-platelet interactions in sepsis', Critical Care Medicine, vol. 27, no. 1 SUPPL..
Aziz M, Kirschenbaum LA, Astiz ME, Saha DC, Rackow EC. Neutrophil-platelet interactions in sepsis. Critical Care Medicine. 1999;27(1 SUPPL.).
Aziz, Mohammed ; Kirschenbaum, Linda A. ; Astiz, Mark E. ; Saha, Dhanonjoy C. ; Rackow, Eric C. / Neutrophil-platelet interactions in sepsis. In: Critical Care Medicine. 1999 ; Vol. 27, No. 1 SUPPL.
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