Neurotrophin-induced transport of a β-actin mRNP complex increases β-actin levels and stimulates growth cone motility

H. L. Zhang, T. Eom, Y. Oleynikov, Shailesh M. Shenoy, D. A. Liebelt, J. B. Dictenberg, Robert H. Singer, G. J. Bassell

Research output: Contribution to journalArticle

272 Citations (Scopus)

Abstract

Neurotrophin regulation of actin-dependent changes in growth cone motility may depend on the signaling of β-actin mRNA transport. Formation of an RNP complex between the β-actin mRNA zipcode sequence and Zipcode Binding Protein 1 (ZBP1) was required for its localization to growth cones. Antisense oligonucleotides to the zipcode inhibited formation of this RNP complex in vitro and the neurotrophin-induced localization of β-actin mRNA and ZBP1 granules. Live cell imaging of neurons transfected with EGFP-ZBP1 revealed fast, bidirectional movements of granules in neurites that were inhibited by antisense treatment, as visualized by FRAP analysis. NT-3 stimulation of β-actin protein localization was dependent on the 3′UTR and inhibited by antisense treatment. Growth cones exhibited impaired motility in the presense of antisense. These results suggest a novel mechanism to influence growth cone dynamics involving the regulated transport of mRNA.

Original languageEnglish (US)
Pages (from-to)261-275
Number of pages15
JournalNeuron
Volume31
Issue number2
DOIs
StatePublished - Aug 2 2001

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Growth Cones
Nerve Growth Factors
Actins
Carrier Proteins
Messenger RNA
Antisense Oligonucleotides
Neurites
messenger ribonucleoprotein
Neurons
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neurotrophin-induced transport of a β-actin mRNP complex increases β-actin levels and stimulates growth cone motility. / Zhang, H. L.; Eom, T.; Oleynikov, Y.; Shenoy, Shailesh M.; Liebelt, D. A.; Dictenberg, J. B.; Singer, Robert H.; Bassell, G. J.

In: Neuron, Vol. 31, No. 2, 02.08.2001, p. 261-275.

Research output: Contribution to journalArticle

Zhang, H. L. ; Eom, T. ; Oleynikov, Y. ; Shenoy, Shailesh M. ; Liebelt, D. A. ; Dictenberg, J. B. ; Singer, Robert H. ; Bassell, G. J. / Neurotrophin-induced transport of a β-actin mRNP complex increases β-actin levels and stimulates growth cone motility. In: Neuron. 2001 ; Vol. 31, No. 2. pp. 261-275.
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