Neuroscience

Neuronal and intestinal protein kinase D isoforms mediate Na+ (salt taste)-induced learning

Ya Fu, Min Ren, Hui Feng, Lu Chen, Zeynep F. Altun, Charles S. Rubin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Ubiquitously expressed protein kinase D (PKD) isoforms are poised to disseminate signals carried by diacylglycerol (DAG). However, the in vivo regulation and functions of PKDs are poorly understood. We show that the Caenorhabditis elegans gene, dkf-2, encodes not just DKF-2A, but also a second previously unknown isoform, DKF-2B. Whereas DKF-2A is present mainly in intestine, we show that DKF-2B is found in neurons. Characterization of dkf-2 null mutants and transgenic animals expressing DKF-2B, DKF-2A, or both isoforms revealed that PKDs couple DAG signals to regulation of sodium ion (Na +)-induced learning. EGL-8 (a phospholipase Cβ4 homolog) and TPA-1 (a protein kinase Cδ homolog) are upstream regulators of DKF-2 isoforms in vivo. Thus, pathways containing EGL-8-TPA-1-DKF-2 enable learning and behavioral plasticity by receiving, transmitting, and cooperatively integrating environmental signals targeted to both neurons and intestine.

Original languageEnglish (US)
JournalScience Signaling
Volume2
Issue number83
DOIs
StatePublished - Aug 11 2009

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Neurosciences
Protein Isoforms
Salts
Learning
Diglycerides
Neurons
Intestines
Genetically Modified Animals
Phospholipases
Caenorhabditis elegans
Protein Kinase C
Plasticity
Animals
Genes
Sodium
Ions
protein kinase D

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Neuroscience : Neuronal and intestinal protein kinase D isoforms mediate Na+ (salt taste)-induced learning. / Fu, Ya; Ren, Min; Feng, Hui; Chen, Lu; Altun, Zeynep F.; Rubin, Charles S.

In: Science Signaling, Vol. 2, No. 83, 11.08.2009.

Research output: Contribution to journalArticle

Fu, Ya ; Ren, Min ; Feng, Hui ; Chen, Lu ; Altun, Zeynep F. ; Rubin, Charles S. / Neuroscience : Neuronal and intestinal protein kinase D isoforms mediate Na+ (salt taste)-induced learning. In: Science Signaling. 2009 ; Vol. 2, No. 83.
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