Abstract
Ubiquitously expressed protein kinase D (PKD) isoforms are poised to disseminate signals carried by diacylglycerol (DAG). However, the in vivo regulation and functions of PKDs are poorly understood. We show that the Caenorhabditis elegans gene, dkf-2, encodes not just DKF-2A, but also a second previously unknown isoform, DKF-2B. Whereas DKF-2A is present mainly in intestine, we show that DKF-2B is found in neurons. Characterization of dkf-2 null mutants and transgenic animals expressing DKF-2B, DKF-2A, or both isoforms revealed that PKDs couple DAG signals to regulation of sodium ion (Na +)-induced learning. EGL-8 (a phospholipase Cβ4 homolog) and TPA-1 (a protein kinase Cδ homolog) are upstream regulators of DKF-2 isoforms in vivo. Thus, pathways containing EGL-8-TPA-1-DKF-2 enable learning and behavioral plasticity by receiving, transmitting, and cooperatively integrating environmental signals targeted to both neurons and intestine.
| Original language | English (US) |
|---|---|
| Journal | Science Signaling |
| Volume | 2 |
| Issue number | 83 |
| DOIs | |
| State | Published - Aug 11 2009 |
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ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology
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Neuroscience : Neuronal and intestinal protein kinase D isoforms mediate Na+ (salt taste)-induced learning. / Fu, Ya; Ren, Min; Feng, Hui; Chen, Lu; Altun, Zeynep F.; Rubin, Charles S.
In: Science Signaling, Vol. 2, No. 83, 11.08.2009.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neuroscience
T2 - Neuronal and intestinal protein kinase D isoforms mediate Na+ (salt taste)-induced learning
AU - Fu, Ya
AU - Ren, Min
AU - Feng, Hui
AU - Chen, Lu
AU - Altun, Zeynep F.
AU - Rubin, Charles S.
PY - 2009/8/11
Y1 - 2009/8/11
N2 - Ubiquitously expressed protein kinase D (PKD) isoforms are poised to disseminate signals carried by diacylglycerol (DAG). However, the in vivo regulation and functions of PKDs are poorly understood. We show that the Caenorhabditis elegans gene, dkf-2, encodes not just DKF-2A, but also a second previously unknown isoform, DKF-2B. Whereas DKF-2A is present mainly in intestine, we show that DKF-2B is found in neurons. Characterization of dkf-2 null mutants and transgenic animals expressing DKF-2B, DKF-2A, or both isoforms revealed that PKDs couple DAG signals to regulation of sodium ion (Na +)-induced learning. EGL-8 (a phospholipase Cβ4 homolog) and TPA-1 (a protein kinase Cδ homolog) are upstream regulators of DKF-2 isoforms in vivo. Thus, pathways containing EGL-8-TPA-1-DKF-2 enable learning and behavioral plasticity by receiving, transmitting, and cooperatively integrating environmental signals targeted to both neurons and intestine.
AB - Ubiquitously expressed protein kinase D (PKD) isoforms are poised to disseminate signals carried by diacylglycerol (DAG). However, the in vivo regulation and functions of PKDs are poorly understood. We show that the Caenorhabditis elegans gene, dkf-2, encodes not just DKF-2A, but also a second previously unknown isoform, DKF-2B. Whereas DKF-2A is present mainly in intestine, we show that DKF-2B is found in neurons. Characterization of dkf-2 null mutants and transgenic animals expressing DKF-2B, DKF-2A, or both isoforms revealed that PKDs couple DAG signals to regulation of sodium ion (Na +)-induced learning. EGL-8 (a phospholipase Cβ4 homolog) and TPA-1 (a protein kinase Cδ homolog) are upstream regulators of DKF-2 isoforms in vivo. Thus, pathways containing EGL-8-TPA-1-DKF-2 enable learning and behavioral plasticity by receiving, transmitting, and cooperatively integrating environmental signals targeted to both neurons and intestine.
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U2 - 10.1126/scisignal.2000224
DO - 10.1126/scisignal.2000224
M3 - Article
C2 - 19671928
AN - SCOPUS:73349131743
VL - 2
JO - Science Signaling
JF - Science Signaling
SN - 1937-9145
IS - 83
ER -